Barth A, Klinger W, Hoppe H
Arch Int Pharmacodyn Ther. 1986 Sep;283(1):16-29.
Maturation and phenobarbital inducibility of hepatic uptake of the organic anions indocyanine green (ICG) and eosine were studied in rats of different ages. Bile duct ligation reduced the ICG and eosine liver concentration in young rats. Therefore, bile duct ligation cannot be recommended for uptake investigations in rats of different ages. The longest elimination half life time (12.4 min) was found in 10-day-old rats within the first 15 min after i.v. administration of 38 mumol ICG/kg. In 20-day-old rats the ICG half life was significantly shorter than in adult 110-day-old rats (5.8 versus 9.6 min). Phenobarbital pretreatment did not alter the ICG elimination half lives in 10-, 20- and 110-day-old rats. The shortest elimination half life time of eosine was found in 60-day-old rats (33.0 min versus 80.3 min in 10-day-old rats and 62.4 min in 240-day-old ones). Phenobarbital pretreatment did not influence eosine serum concentrations. Our results indicate for ICG that uptake comes to maturity earlier than biliary excretion. Differences between ICG and eosine in maturation of the two transport steps support the assumption of more than one carrier for exogenous organic anions. Dye uptake was not altered after phenobarbital and, consequently, cannot be responsible for enhanced biliary dye excretion in differently aged rats.
研究了不同年龄大鼠肝脏对有机阴离子吲哚菁绿（ICG）和伊红摄取的成熟度及苯巴比妥诱导性。胆管结扎降低了幼龄大鼠肝脏中ICG和伊红的浓度。因此，不建议在不同年龄的大鼠中进行胆管结扎来进行摄取研究。静脉注射38 μmol ICG/kg后15分钟内，10日龄大鼠的消除半衰期最长（12.4分钟）。20日龄大鼠的ICG半衰期明显短于成年110日龄大鼠（5.8分钟对9.6分钟）。苯巴比妥预处理未改变10日龄、20日龄和110日龄大鼠的ICG消除半衰期。伊红的最短消除半衰期出现在60日龄大鼠中（33.0分钟，而10日龄大鼠为80.3分钟，240日龄大鼠为62.4分钟）。苯巴比妥预处理不影响伊红血清浓度。我们的结果表明，对于ICG来说，摄取比胆汁排泄更早成熟。ICG和伊红在两个转运步骤成熟度上的差异支持了外源性有机阴离子存在多种载体的假设。苯巴比妥处理后染料摄取未改变，因此，它不可能是不同年龄大鼠胆汁染料排泄增加的原因。
