Department of Paraclinical Sciences, Phytomedicine Programme, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa.
BMC Complement Altern Med. 2013 Dec 13;13:356. doi: 10.1186/1472-6882-13-356.
Athrixia phylicoides DC. (Asteraceae) is used medicinally in South Africa to treat a plethora of ailments, including heart problems, diabetes, diarrhoea, sores and infected wounds. It is also prepared in the form of a tea (hot decoction) taken as a refreshing, pleasant-tasting beverage with commercialization potential.
Extracts of the dried ground aerial parts were prepared using organic solvents (diethyl ether, dichloromethane/methanol, ethyl acetate and ethanol) and water. These extracts were subjected to HPLC, TLC and bioautography analysis with the aim of linking a range of peaks visualized in HPLC chromatography profiles to antibacterial and antifungal activity of the same extracts.
HPLC revealed a group of compounds extracted by more than one solvent. Compounds identified include inositol, caffeic acid, quercetin, kaempferol, apigenin, hymenoxin and oleanolic acid. The organic extracts displayed similar TLC profiles, and bioautography indicated approximately five antibacterial compounds, but only two antifungal compounds in these extracts. Bioautography indicated that cold water extracted the least antimicrobial compounds.
Several previously unknown compounds were identified in Athrixia phylicoides extracts, and bioautography indicated a number of antibacterial and antifungal compounds. There were notable differences in chemical composition and bioactivity between the organic and aqueous extracts. Further research is necessary to fully characterize the active components of the extracts.
Athrixia phylicoides DC.（菊科）在南非被用作药物治疗多种疾病，包括心脏问题、糖尿病、腹泻、溃疡和感染伤口。它也被制成茶（热浸剂），作为一种清爽、口感好的饮料，具有商业化潜力。
用有机溶剂（乙醚、二氯甲烷/甲醇、乙酸乙酯和乙醇）和水制备干燥的地上部分的提取物。对这些提取物进行 HPLC、TLC 和生物自显影分析，目的是将 HPLC 色谱图中观察到的一系列峰与相同提取物的抗菌和抗真菌活性联系起来。
HPLC 显示了一组用多种溶剂提取的化合物。鉴定的化合物包括肌醇、咖啡酸、槲皮素、山奈酚、芹菜素、 hymenoxin 和齐墩果酸。有机提取物显示出相似的 TLC 图谱，生物自显影表明这些提取物中大约有五种抗菌化合物，但只有两种抗真菌化合物。生物自显影表明，冷水提取的抗菌化合物最少。
从 Athrixia phylicoides 提取物中鉴定出几种以前未知的化合物，生物自显影表明存在多种抗菌和抗真菌化合物。有机溶剂和水提取物之间在化学成分和生物活性方面存在显著差异。需要进一步研究以充分表征提取物的活性成分。
