养殖牙鲆鱼源肽对斑马鱼氧化应激的保护作用。
Protective effect of aquacultured flounder fish-derived peptide against oxidative stress in zebrafish.
机构信息
Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
Industry-Academy Cooperation Foundation, Jeju National University, Jeju 690-756, Republic of Korea.
出版信息
Fish Shellfish Immunol. 2014 Jan;36(1):320-3. doi: 10.1016/j.fsi.2013.11.018. Epub 2013 Dec 11.
This study investigates the protective effect of aquacultured flounder fish-derived peptide (AFFP) against 2,2-azobis-(2-amidinopropane) hydrochloride (AAPH)-induced oxidative damage in a zebrafish model. Zebrafish embryos were evaluated for the protective effect by heartbeat rate, survival rate, ROS generation, lipid peroxidation, and cell death. In the results, the AAPH group showed a low survival rate, whereas the AFFP and AAPH co-treated group increased a survival rate. Also, AFFP dose-dependently reduced AAPH-induced intracellular ROS and lipid peroxidation, and decreased cell death in AAPH-induced zebrafish. These results revealed that AFFP could be used as a natural antioxidant, and that the zebrafish provides an alternative in vivo model to efficiently evaluate the antioxidative effects of peptides on fishes.
本研究探讨了养殖牙鲆鱼衍生肽（AFFP）对 2,2-偶氮双（2-脒基丙烷）盐酸盐（AAPH）诱导的斑马鱼模型氧化损伤的保护作用。通过心跳率、存活率、ROS 生成、脂质过氧化和细胞死亡来评估斑马鱼胚胎的保护作用。结果显示，AAPH 组存活率较低，而 AFFP 和 AAPH 共同处理组存活率增加。此外，AFFP 剂量依赖性地降低了 AAPH 诱导的细胞内 ROS 和脂质过氧化，并减少了 AAPH 诱导的斑马鱼中的细胞死亡。这些结果表明，AFFP 可用作天然抗氧化剂，并且斑马鱼提供了一种替代的体内模型，可有效地评估肽对鱼类的抗氧化作用。
Zotero 插件