Department of Rheumatology, Leiden University Medical Center, , Leiden, The Netherlands.
Ann Rheum Dis. 2014 Apr;73(4):780-3. doi: 10.1136/annrheumdis-2013-204154. Epub 2013 Dec 13.
The presence of anti-citrullinated protein antibodies (ACPA) and IgM-rheumatoid factor (IgM-RF) years before the clinical diagnosis of rheumatoid arthritis (RA) suggests they are possibly involved in the pathogenic process underlying RA. In this study, we analysed whether anti-carbamylated protein (anti-CarP) antibodies, a novel autoantibody system against carbamylated proteins, can also be detected in healthy individuals before they developed RA.
Multiple sera from asymptomatic blood donors prior to the onset of their RA symptoms and sera from age-matched and sex-matched controls were tested for the presence of antibodies directed against carbamylated-fetal calf serum (Ca-FCS), carbamylated-fibrinogen (Ca-Fib), cyclic citrullinated-peptide 2 and IgM-RF.
Anti-Ca-FCS and anti-Ca-Fib antibodies were each present in 27% and 38% of the last serum samples of blood donors prior to the diagnosis of RA. Both anti-Ca-FCS and anti-Ca-Fib antibodies could be detected many years before the onset of RA. Anti-CarP antibodies as well as ACPA are, on average, detected earlier than IgM-RF.
In addition to ACPA and IgM-RF, also the newly identified anti-CarP antibodies appear many years before the diagnosis of RA.
抗瓜氨酸化蛋白抗体（ACPA）和 IgM 类风湿因子（IgM-RF）在类风湿关节炎（RA）临床诊断前数年出现，表明它们可能参与了 RA 的发病机制。本研究分析了在发生 RA 之前，是否也能在健康个体中检测到针对瓜氨酸化蛋白的新型自身抗体系统——抗碳化蛋白（anti-CarP）抗体。
在出现 RA 症状之前，对无症状献血者的多份血清和年龄及性别匹配的对照者的血清进行了针对碳化胎牛血清（Ca-FCS）、碳化纤维蛋白原（Ca-Fib）、环瓜氨酸肽 2 和 IgM-RF 的抗体检测。
在 RA 诊断前的最后一份血清样本中，抗 Ca-FCS 和抗 Ca-Fib 抗体分别存在于 27%和 38%的献血者中。抗 Ca-FCS 和抗 Ca-Fib 抗体都可以在 RA 发病前多年被检测到。抗 CarP 抗体以及 ACPA 的检测时间平均比 IgM-RF 更早。
除 ACPA 和 IgM-RF 外，新发现的抗 CarP 抗体也在 RA 诊断前多年被检测到。
