类风湿关节炎患者未患病的一级亲属中的抗氨甲酰化蛋白抗体：与抗环瓜氨酸化蛋白抗体和类风湿因子缺乏相关性。

Anti-carbamylated protein antibodies in unaffected first-degree relatives of rheumatoid arthritis patients: lack of correlation with anti-cyclic citrullinated protein antibodies and rheumatoid factor.

作者信息

Alessandri C, Bartosiewicz I, Pendolino M, Mancini R, Colasanti T, Pecani A, Morello F, Mastrangelo A, Sabatinelli D, Riccieri V, Di Franco M, Ceccarelli F, Perricone C, Conti F, Valesini G

机构信息

Dipartimento di Medicina Interna e Specialità Mediche, Sapienza Università di Roma, Italy.

出版信息

Clin Exp Rheumatol. 2015 Nov-Dec;33(6):824-30. Epub 2015 Sep 28.

PMID:26411477

Abstract

OBJECTIVES

To investigate the prevalence of anti-carbamylated protein antibodies (anti-CarP) in the healthy first-degree relatives (HFDRs) of patients with rheumatoid arthritis (RA).

METHODS

We enrolled 141 HFDRs of 63 patients with RA diagnosed accordingly to the 2010 ACR/EULAR criteria. Fifty-six normal healthy subjects (NHS), sex- and age-matched, served as controls. Anti-CarP IgG, anti-cyclic citrullinated peptide antibody (anti-CCP) IgG and rheumatoid factors (RF) isotypes (IgG, IgA, IgM) were assessed by solid-phase ELISA.

RESULTS

Anti-CarP were detectable in 13 HFDRs (9.2%), anti-CCP in 9 (6.3%), IgG-RF in 10 (7%), IgA-RF in 17 (12%), and IgM-RF in 13 (9.2%) HFDRs. Twenty-nine (46%) RA patients were positive for anti-CarP, 31 (49.2%) for anti-CCP, and 34 (53.9%) for RF. One NHS (1.7%) resulted positive for anti-CarP, none for anti-CCP and RF. Anti-CarP showed significantly higher serum levels in RA and HFDRs than in NHS (p<0.0001 and p=0.0012, respectively). A significant correlation between anti-CCP and RF were found among RA patients (p=0.0002), whereas no correlations were reported between autoantibodies tested in the HFDRs.

CONCLUSIONS

Anti-CarP can be found in the sera of HFDRs of RA patients and their prevalence is significantly higher than in NHS. No correlation of anti-CarP with anti-CCP and RF antibodies in RA HFDRs was found.

摘要

目的

调查类风湿关节炎（RA）患者的健康一级亲属（HFDRs）中抗氨甲酰化蛋白抗体（抗CarP）的患病率。

方法

我们纳入了63例根据2010年美国风湿病学会（ACR）/欧洲抗风湿病联盟（EULAR）标准确诊的RA患者的141名HFDRs。56名年龄和性别匹配的正常健康受试者（NHS）作为对照。通过固相酶联免疫吸附测定法评估抗CarP IgG、抗环瓜氨酸肽抗体（抗CCP）IgG和类风湿因子（RF）各亚型（IgG、IgA、IgM）。

结果

在13名HFDRs（9.2%）中可检测到抗CarP，9名（6.3%）中可检测到抗CCP，10名（7%）中可检测到IgG-RF，17名（12%）中可检测到IgA-RF，13名（9.2%）中可检测到IgM-RF。29名（46%）RA患者抗CarP呈阳性，31名（49.2%）抗CCP呈阳性，34名（53.9%）RF呈阳性。1名NHS（1.7%）抗CarP呈阳性，抗CCP和RF均为阴性。RA患者和HFDRs中抗CarP的血清水平显著高于NHS（分别为p<0.0001和p=0.0012）。在RA患者中发现抗CCP与RF之间存在显著相关性（p=0.0002），而在HFDRs中检测的自身抗体之间未报告相关性。