Department of Biochemistry, North-Eastern Hill University, Shillong, 793022, India.
Cell Mol Neurobiol. 2014 Mar;34(2):307-13. doi: 10.1007/s10571-013-0015-8. Epub 2013 Dec 12.
Oxidative stress is an important factor in causing aging and age-related diseases. It is caused by an imbalance between oxidants such as reactive oxygen species (ROS) and antioxidants. Protein oxidation elicited by free radicals may cause protein function disruptions. Protein carbonylation, an irreversible process resulting in loss of function of the modified proteins, is a widely used marker for oxidative stress. In the present study, we have evaluated the levels of protein carbonyls, ROS, and catalase in the cerebral hemispheres of young and aged mice. When aged mice were subjected to a dietary restriction (DR) regimen (alternate days feeding) of 3 months, a significant reduction in the endogenous levels of protein carbonylation as well as ROS and elevation of catalase was observed in their cerebral hemispheres. The present study, thus, demonstrated the antioxidative effects of late-onset DR regimen in the cerebral hemispheres of aged mice which may act as a powerful modulator of age-related neurodegenerative diseases.
氧化应激是导致衰老和与年龄相关疾病的重要因素。它是由氧化剂（如活性氧物种[ROS]）和抗氧化剂之间的不平衡引起的。自由基引起的蛋白质氧化可能导致蛋白质功能紊乱。蛋白质羰基化是一种导致修饰蛋白功能丧失的不可逆过程，是氧化应激的广泛应用标志物。在本研究中，我们评估了年轻和老年小鼠大脑半球中的蛋白质羰基、ROS 和过氧化氢酶水平。当老年小鼠接受为期 3 个月的饮食限制（DR）方案（隔日喂养）时，其大脑半球中的内源性蛋白质羰基水平以及 ROS 和过氧化氢酶水平显著降低。因此，本研究表明，晚期 DR 方案在老年小鼠大脑半球中具有抗氧化作用，可能作为与年龄相关的神经退行性疾病的有力调节剂。
