Jehlička Jan, Oren Aharon
Institute of Geochemistry, Mineralogy and Mineral Resources, Charles University in Prague Prague, Czech Republic.
Front Microbiol. 2013 Dec 10;4:380. doi: 10.3389/fmicb.2013.00380.
Raman spectroscopy plays a major role in robust detection of biomolecules and mineral signatures in halophile research. An overview of Raman spectroscopic investigations in halophile research of the last decade is given here to show advantages of the approach, progress made as well as limits of the technique. Raman spectroscopy is an excellent tool to monitor and identify microbial pigments and other biomolecules in extant and extinct halophile biomass. Studies of bottom gypsum crusts from salterns, native evaporitic sediments, halite inclusions, and endoliths as well as cultures of halophilic microorganisms permitted to understand the content, distribution, and behavior of important molecular species. The first papers describing Raman spectroscopic detection of microbiological and geochemical key markers using portable instruments are highlighted as well.
拉曼光谱在嗜盐菌研究中对生物分子和矿物质特征的可靠检测起着重要作用。本文概述了过去十年嗜盐菌研究中的拉曼光谱研究,以展示该方法的优势、取得的进展以及该技术的局限性。拉曼光谱是监测和识别现存和已灭绝嗜盐菌生物量中微生物色素及其他生物分子的出色工具。对盐田底部石膏壳、原生蒸发沉积物、石盐包裹体、石内微生物以及嗜盐微生物培养物的研究,有助于了解重要分子种类的含量、分布和行为。本文还重点介绍了首批描述使用便携式仪器进行拉曼光谱检测微生物和地球化学关键标志物的论文。