Institute for Integrated Micro and Nano Systems, School of Engineering, The University of Edinburgh, Edinburgh EH9 3JF, United Kingdom.
Biomicrofluidics. 2012 Dec 12;6(4):44113. doi: 10.1063/1.4771316. eCollection 2012.
Assessment of the dielectrophoresis (DEP) cross-over frequency (f xo), cell diameter, and derivative membrane capacitance (C m) values for a group of undifferentiated human embryonic stem cell (hESC) lines (H1, H9, RCM1, RH1), and for a transgenic subclone of H1 (T8) revealed that hESC lines could not be discriminated on their mean f xo and C m values, the latter of which ranged from 14 to 20 mF/m(2). Differentiation of H1 and H9 to a mesenchymal stem cell-like phenotype resulted in similar significant increases in mean C m values to 41-49 mF/m(2) in both lines (p < 0.0001). BMP4-induced differentiation of RCM1 to a trophoblast cell-like phenotype also resulted in a distinct and significant increase in mean C m value to 28 mF/m(2) (p < 0.0001). The progressive transition to a higher membrane capacitance was also evident after each passage of cell culture as H9 cells transitioned to a mesenchymal stem cell-like state induced by growth on a substrate of hyaluronan. These findings confirm the existence of distinctive parameters between undifferentiated and differentiating cells on which future application of dielectrophoresis in the context of hESC manufacturing can be based.
对一组未分化的人类胚胎干细胞（hESC）系（H1、H9、RCM1、RH1）和 H1 的一个转基因亚克隆（T8）的介电泳（DEP）交叉频率（f xo）、细胞直径和衍生膜电容（C m）值进行评估后发现，hESC 系不能根据它们的平均 f xo 和 C m 值来区分，后者的范围为 14 至 20 mF/m(2)。H1 和 H9 向间充质干细胞样表型的分化导致 C m 值的平均显著增加到 41-49 mF/m(2)（p < 0.0001）。BMP4 诱导 RCM1 向滋养层样细胞表型的分化也导致 C m 值的明显和显著增加到 28 mF/m(2)（p < 0.0001）。随着细胞培养的每一次传代，膜电容的逐渐增加也很明显，因为 H9 细胞在透明质酸基质上生长诱导的间充质干细胞样状态下发生转变。这些发现证实了未分化和分化细胞之间存在独特的参数，未来可以在 hESC 制造的背景下应用介电泳。
