Walker J, Shadanbaz S, Woodfield T B F, Staiger M P, Dias G J
Department of Anatomy and Structural Biology, University of Otago, Dunedin, New Zealand.
Biomed Mater. 2014 Feb;9(1):015006. doi: 10.1088/1748-6041/9/1/015006. Epub 2013 Dec 16.
Magnesium (Mg) and its alloys are being widely investigated for their potential use as resorbable biomaterials for orthopaedic applications. However, the natural corrosion of the metals results in potentially harmful perturbations to the physiological environment, which requires a comprehensive understanding of their biocompatibility. Currently, most investigations proceed directly from in vitro biocompatibility studies to intraosseous implantation. However, this can result in the unnecessary elimination of appropriate materials due to over sensitive in vitro methods or the implantation of potentially harmful materials. This study involved the development of a relevant in vitro cell culture method, and an in vivo soft tissue implantation technique to provide an intermediate step between basic cell culture methods and large animal intraosseous investigations. A Live/Dead fluorescent assay was used to investigate the viability of both L929 and SaOS-2 cells exposed to Mg alloys, with the results compared to those seen with the intramuscular implantation of the same materials in Lewis rats. These methods were able to successfully provide data on the corrosion of Mg alloys, allowing the identification of slowly and safely corroding materials that may be used in future intraosseous investigations.
镁（Mg）及其合金作为可吸收生物材料在骨科应用中的潜在用途正受到广泛研究。然而，金属的自然腐蚀会对生理环境造成潜在的有害干扰，这就需要全面了解它们的生物相容性。目前，大多数研究直接从体外生物相容性研究进入骨内植入。然而，这可能会因体外方法过于敏感而导致合适材料被不必要地淘汰，或者植入潜在有害的材料。本研究开发了一种相关的体外细胞培养方法和一种体内软组织植入技术，以在基础细胞培养方法和大型动物骨内研究之间提供一个中间步骤。采用活/死荧光检测法研究暴露于镁合金的L929和SaOS-2细胞的活力，并将结果与相同材料在Lewis大鼠肌肉内植入的结果进行比较。这些方法能够成功提供有关镁合金腐蚀的数据，从而识别出可用于未来骨内研究的缓慢且安全腐蚀的材料。
