Arlian L G, Bernstein I L, Vyszenski-Moher D L, Gallagher J S
J Allergy Clin Immunol. 1987 Mar;79(3):467-76. doi: 10.1016/0091-6749(87)90364-2.
Heterologous crossed immunoelectrophoresis (CIE) analysis demonstrated that Dermatophagoides pteronyssinus body and D. farinae body extracts contained up to 21 cross-reacting antigens (Ags). Incubation of CIE gels with two mite-sensitive individual sera and a serum pool of mite-sensitive patients indicated that up to nine cross-reacting Ags bound mite-specific IgE on crossed radioimmunoelectrophoresis and were common allergens. Likewise, heterologous CIE analysis of D. pteronyssinus feces and D. farinae feces extracts demonstrated approximately 13 cross-reacting Ags. Up to eight of these cross-reacting fecal Ags bound mite feces-specific human IgE. Additional cross antigenicity and allergenicity was evident between body extract of one species and feces extract of the other species. The amount of specific IgE binding to cross-reacting Ags in interspecific extracts varied both between and within individual sera. Different cross-reacting Ags contained potent allergens, and these varied from patient to patient. Also of major significance was the fact that body and fecal extracts of each species contained Ags and allergens that were unique and species specific.
异源交叉免疫电泳（CIE）分析表明，屋尘螨虫体和粉尘螨虫体提取物含有多达21种交叉反应抗原（Ag）。用两份对螨敏感的个体血清以及一份螨敏感患者血清池与CIE凝胶温育，结果表明，多达9种交叉反应抗原在交叉放射免疫电泳中与螨特异性IgE结合，属于常见变应原。同样，对屋尘螨粪便和粉尘螨粪便提取物进行异源CIE分析，结果显示约有13种交叉反应抗原。这些交叉反应粪便抗原中多达8种与螨粪便特异性人IgE结合。在一个物种的虫体提取物和另一个物种的粪便提取物之间，额外的交叉抗原性和变应原性很明显。种间提取物中与交叉反应抗原结合的特异性IgE量在个体血清之间和个体血清内均有所不同。不同的交叉反应抗原含有强效变应原，且这些变应原因患者而异。同样重要的是，每个物种的虫体和粪便提取物都含有独特的、物种特异性的抗原和变应原。
