Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, Tamil Nadu, India.
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, Tamil Nadu, India.
Chin J Nat Med. 2013 Nov;11(6):621-7. doi: 10.1016/S1875-5364(13)60072-2.
Considering the importance of diet in the prevention of cellular damage caused by reactive oxygen species which has been implicated for several diseases, this present study was undertaken to evaluate the in vitro and in vivo antioxidant potential of the ethanolic extract of the fruiting bodies of Ganoderma lucidum on 7, 12-dimethylbenz(a)anthracene (DMBA)-induced mammary carcinogenesis in Sprague Dawley rats.
Ganoderma lucidum extract was tested for in vitro antioxidant and radical scavenging assays, such as (ABTS(+)) radical cation decolorization assay, DPPH radical scavenging, hydroxyl radical, and superoxide radical scavenging assays. The in vivo antioxidant potentials were analyzed by SOD, CAT, and GPx in plasma, mammary, and liver tissues.
In all the in vitro antioxidant and radical scavenging assays the extract exhibited good scavenging activity. In vivo enzymatic antioxidant levels, such as SOD, CAT, and GPx were decreased in DMBA-induced animals. Moreover, pretreatment with G. lucidum (500 mg · kg(-1) bw) to DMBA-induced animals significantly (P < 0.05) increased the levels of SOD, CAT, and GPx in plasma, mammary, and liver tissues compared to DMBA induced animals.
From these findings, it is suggested that G. lucidum extract could be considered as a potential source of natural antioxidants and can be used as an effective chemopreventive agent against mammary cancer.
考虑到饮食在预防活性氧引起的细胞损伤中的重要性，而活性氧与许多疾病的发生有关，本研究旨在评估灵芝子实体的乙醇提取物在 DMBA 诱导的 Sprague Dawley 大鼠乳腺致癌中的体外和体内抗氧化潜力。
对灵芝提取物进行了体外抗氧化和自由基清除试验，如 ABTS(+)自由基阳离子褪色试验、DPPH 自由基清除试验、羟基自由基和超氧自由基清除试验。通过测定血浆、乳腺和肝脏组织中的 SOD、CAT 和 GPx 来分析体内抗氧化潜力。
在所有体外抗氧化和自由基清除试验中，提取物均表现出良好的清除活性。DMBA 诱导的动物体内的 SOD、CAT 和 GPx 等酶抗氧化水平降低。此外，与 DMBA 诱导的动物相比，用灵芝（500mg·kg(-1)bw）预处理 DMBA 诱导的动物可显著提高（P<0.05）血浆、乳腺和肝脏组织中的 SOD、CAT 和 GPx 水平。
从这些发现可以看出，灵芝提取物可以被认为是天然抗氧化剂的潜在来源，并可作为一种有效的化学预防乳腺肿瘤的药物。
