Cilerdžić Jasmina, Vukojević Jelena, Stajić Mirjana, Stanojković Tatjana, Glamočlija Jasmina
University of Belgrade, Faculty of Biology, Takovska 43, 11000 Belgrade, Serbia.
University of Belgrade, Faculty of Biology, Takovska 43, 11000 Belgrade, Serbia.
J Ethnopharmacol. 2014 Aug 8;155(1):312-9. doi: 10.1016/j.jep.2014.05.036. Epub 2014 May 29.
Ganoderma lucidum (W. Curt.:Fr.) P. Karst. has been used for several thousand years in traditional medicine of the Far East for the treatment of a various diseases including: cancer, immunological disorders, hepatitis, arthritis, nephritis, bronchitis, asthma etc. Ganoderma species are natural sources of various antimicrobial, antioxidant and antitumor active compounds which can be alternative to commercial antibiotics, antimycotics, antioxidants and cytostatics. As the amount of wild Ganoderma lucidum basidiocarps is not sufficient, the aim of this study was to compare the bioactivity of basidiaocarps obtained on alternative substrate (wheat straw) with commercially cultivated ones.
Antimicrobial potential of the tested Ganoderma lucidum extracts was studied by disc-diffusion and microdilution method. Cytotoxic effect of tested extracts on two cancer cell lines was determined by microculture tetrazolium test. DPPH scavenging ability, total phenols and flavonoids content were measured in order to determine the antioxidative potential of Ganoderma lucidum extracts.
Ganoderma lucidum BEOFB 434 extract was the most effective antibacterial agent, while commercial strain was the weakest. DPPH· scavenging activity of extracts of basidiocarps from alternative substrate was also significantly higher than that of commercially obtained basidiocarps. The extracts of Ganoderma lucidum BEOFB 431 exhibited the strongest cytotoxic activity significantly higher than those in commercial strain.
The study showed that fruiting bodies produced on one of the most accessible and cheapest crop residue, wheat straw, are better antimicrobial, antioxidant and cytotoxic agents than those obtained on conventional substrate, oak sawdust.
灵芝（Ganoderma lucidum (W. Curt.:Fr.) P. Karst.）在远东传统医学中已被使用了数千年，用于治疗多种疾病，包括癌症、免疫紊乱、肝炎、关节炎、肾炎、支气管炎、哮喘等。灵芝种类是各种抗菌、抗氧化和抗肿瘤活性化合物的天然来源，可替代商业抗生素、抗真菌剂、抗氧化剂和细胞抑制剂。由于野生灵芝子实体的数量不足，本研究的目的是比较在替代基质（麦秸）上获得的子实体与商业栽培子实体的生物活性。
通过纸片扩散法和微量稀释法研究了受试灵芝提取物的抗菌潜力。通过微量培养四氮唑试验测定受试提取物对两种癌细胞系的细胞毒性作用。测定了DPPH清除能力、总酚和黄酮含量，以确定灵芝提取物的抗氧化潜力。
灵芝BEOFB 434提取物是最有效的抗菌剂，而商业菌株是最弱的。来自替代基质的子实体提取物的DPPH·清除活性也显著高于商业获得的子实体。灵芝BEOFB 431提取物表现出最强的细胞毒性活性，显著高于商业菌株。
该研究表明，在最容易获得且最便宜的作物残渣之一麦秸上产生的子实体，比在传统基质橡木锯末上获得的子实体具有更好的抗菌、抗氧化和细胞毒性作用。
