Vallisuta Omboon, Nukoolkarn Veena, Mitrevej Ampol, Sarisuta Narong, Leelapornpisid Pimporn, Phrutivorapongkul Ampai, Sinchaipanid Nuttanan
Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand.
Department of Manufacturing Pharmacy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand.
Exp Ther Med. 2014 Jan;7(1):246-250. doi: 10.3892/etm.2013.1373. Epub 2013 Oct 30.
Marigold ( L.) has long been used as a medicinal herb for a number of therapeutic activities. In the present study, the cytotoxicities of ethanol and ethyl acetate extracts of marigold flowers and their inhibitory effects on elastase and tyrosinase enzymes were investigated. An MTT assay was performed to measure the cytotoxicity of these two extracts on the H460 lung cancer and the Caco-2 colon cancer cell lines. An elastase assay kit, based on the digestion of a non-fluorescent elastin substrate to highly fluorescent fragments by elastase, was used for the elastase inhibition assay. Tyrosinase inhibition activity was investigated using the dopachrome method with L-3,4-dihydroxyphenylalanine (L-DOPA) as a substrate. The data obtained in this study demonstrated that the extracts were nontoxic to H460 and Caco-2 cell lines. The elastase inhibition activities of ethanol (250 μg/ml) and ethyl acetate (125 μg/ml) extracts were found to be significantly higher than that of the negative control. The tyrosinase inhibition activities of ethanol and ethyl acetate extracts, in terms of the mean inhibition concentration (IC), were 1,078 and 1,467 μg/ml, respectively. To the best of our knowledge, the present study has demonstrated for the first time that marigold flower extracts possess tyrosinase inhibition activity. The activities of ethanol and ethyl acetate extracts of marigold flowers were investigated and indicated that these extracts possess useful properties that may be of interest for cosmetic development.
金盏花（L.）长期以来一直被用作具有多种治疗活性的草药。在本研究中，研究了金盏花花的乙醇提取物和乙酸乙酯提取物的细胞毒性及其对弹性蛋白酶和酪氨酸酶的抑制作用。进行MTT试验以测量这两种提取物对H460肺癌细胞系和Caco-2结肠癌细胞系的细胞毒性。基于弹性蛋白酶将非荧光弹性蛋白底物消化成高荧光片段，使用弹性蛋白酶检测试剂盒进行弹性蛋白酶抑制试验。以L-3,4-二羟基苯丙氨酸（L-DOPA）为底物，采用多巴色素法研究酪氨酸酶抑制活性。本研究获得的数据表明，这些提取物对H460和Caco-2细胞系无毒。发现乙醇提取物（250μg/ml）和乙酸乙酯提取物（125μg/ml）的弹性蛋白酶抑制活性明显高于阴性对照。就平均抑制浓度（IC）而言，乙醇提取物和乙酸乙酯提取物的酪氨酸酶抑制活性分别为1078μg/ml和1467μg/ml。据我们所知，本研究首次证明金盏花花提取物具有酪氨酸酶抑制活性。对金盏花花的乙醇提取物和乙酸乙酯提取物的活性进行了研究，结果表明这些提取物具有有用的特性，可能对化妆品开发具有重要意义。
