Chintong Sutasinee, Phatvej Wipaporn, Rerk-Am Ubon, Waiprib Yaowapha, Klaypradit Wanwimol
Department of Fishery Products, Faculty of Fisheries, Kasetsart University, Bangkok 10900, Thailand.
Expert Center of Innovative Herbal Products, Thailand Institute of Scientific and Technological Research, Pathum Thani 12120, Thailand.
Antioxidants (Basel). 2019 May 13;8(5):128. doi: 10.3390/antiox8050128.
Astaxanthin is a potent antioxidant compared with vitamins and other antioxidants. However, astaxanthin extract from shrimp processing waste has not yet been used in cosmetic products. This study aimed to explore the natural astaxanthin from shrimp shells for antioxidant and antityrosinase activities as well as potential toxicity. The antioxidant activities were performed with 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging, β-carotene bleaching, and singlet oxygen quenching assays. The results revealed that astaxanthin extract demonstrated potent antioxidant activities against DPPH and ABTS radicals, and prevented the bleaching of β-carotene and quenching of singlet oxygen (EC 17.5 ± 3.6, 7.7 ± 0.6, 15.1 ± 1.9 and 9.2 ± 0.5 μg/mL, respectively). Furthermore, the astaxanthin extract could inhibit tyrosinase activity (IC 12.2 ± 1.5 μg/mL) and had no toxic effects on human dermal fibroblast cells. These results suggested that shrimp astaxanthin would be a promising dietary supplement for skin health applications.
与维生素和其他抗氧化剂相比，虾青素是一种强效抗氧化剂。然而，从虾加工废料中提取的虾青素尚未用于化妆品中。本研究旨在探索虾壳中的天然虾青素的抗氧化、抗酪氨酸酶活性以及潜在毒性。通过2,2-二苯基-1-苦基肼（DPPH）、2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基清除、β-胡萝卜素漂白和单线态氧猝灭试验来测定抗氧化活性。结果表明，虾青素提取物对DPPH和ABTS自由基具有强效抗氧化活性，并能防止β-胡萝卜素的漂白和单线态氧的猝灭（EC分别为17.5±3.6、7.7±0.6、15.1±1.9和9.2±0.5μg/mL）。此外，虾青素提取物可抑制酪氨酸酶活性（IC为12.2±1.5μg/mL），且对人皮肤成纤维细胞无毒性作用。这些结果表明，虾虾青素有望成为一种用于皮肤健康的膳食补充剂。
