Nakagawa Y, Perentes E, Rubinstein L J
Acta Neuropathol. 1986;72(1):15-22. doi: 10.1007/BF00687942.
Twenty-eight oligodendrogliomas and seven oligoastrocytomas were immunotested by the peroxidase-antiperoxidase (PAP) method with antiglial fibrillary acidic protein (GFAP) serum, anti-Leu 7 monoclonal antibody (Mab), anti-myelin-associated glycoprotein (MAG) Mab, anti-myelin basic protein (MBP) serum, anti-carbonic anhydrase C (CA C) serum and anti-neuron-specific enolase (NSE) serum. The immunoreactivity of their vascular pattern was studied with Ulex europaeus type I lectin (UEA I). According to their morphology and distribution GFAP-positive cells were respectively interpreted as reactive astrocytes, neoplastic astrocytes and neoplastic oligodendrocytes. Reactive astrocytes were found in the tumor, around the tumor and surrounding the supporting blood vessels. Neoplastic astrocytes were mainly found in the oligoastrocytomas and usually closely intermingled with neoplastic oligodendrocytes. GFAP-positive neoplastic oligodendrocytes were found in the typical oligodendrogliomatous areas. They had central nuclei and GFA positivity was mainly found in the perinuclear cytoplasm. They correspond to the "gliofibrillary oligodendrocytes" described by Herpers and Budka. Of the oligodendrogliomas 91% displayed Leu 7 positivity, but anti-Leu 7 cannot be considered as a specific marker for oligodendrogliomas since other neuroepithelial tumors have been reported to react with this antibody. MAG-, CA C- and NSE-positivities were found in a number of tumor cells in a few oligodendrogliomas. All the tumor cells were MBP-negative, but myelin sheaths and fragments of myelin in the infiltrated white matter were clearly demonstrated by this antiserum. UEA I strikingly demonstrated the vascular pattern of the tumors, and its usefulness as a discriminating marker for the supportive endothelial cells was confirmed.
采用过氧化物酶 - 抗过氧化物酶（PAP）法，用抗胶质纤维酸性蛋白（GFAP）血清、抗Leu 7单克隆抗体（Mab）、抗髓鞘相关糖蛋白（MAG）Mab、抗髓鞘碱性蛋白（MBP）血清、抗碳酸酐酶C（CA C）血清和抗神经元特异性烯醇化酶（NSE）血清，对28例少突胶质细胞瘤和7例少突星形细胞瘤进行免疫检测。用荆豆凝集素I（UEA I）研究其血管模式的免疫反应性。根据GFAP阳性细胞的形态和分布，分别将其解释为反应性星形胶质细胞、肿瘤性星形胶质细胞和肿瘤性少突胶质细胞。在肿瘤内、肿瘤周围及支持血管周围发现反应性星形胶质细胞。肿瘤性星形胶质细胞主要见于少突星形细胞瘤，通常与肿瘤性少突胶质细胞紧密混合。在典型的少突胶质细胞瘤区域发现GFAP阳性的肿瘤性少突胶质细胞。它们有中央核，GFA阳性主要见于核周细胞质。它们与Herpers和Budka描述的“神经胶质纤维性少突胶质细胞”相对应。在少突胶质细胞瘤中，91%显示Leu 7阳性，但抗Leu 7不能被视为少突胶质细胞瘤的特异性标志物，因为据报道其他神经上皮肿瘤也可与该抗体发生反应。在少数少突胶质细胞瘤的一些肿瘤细胞中发现MAG、CA C和NSE阳性。所有肿瘤细胞均为MBP阴性，但该抗血清能清楚地显示浸润白质中的髓鞘和髓鞘碎片。UEA I显著显示了肿瘤的血管模式，并证实了其作为支持性内皮细胞鉴别标志物的有效性。
