Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, Key Laboratory of Urban Agriculture (North), Ministry of Agriculture, Beijing, 100097, China.
Curr Microbiol. 2014 Apr;68(4):536-42. doi: 10.1007/s00284-013-0510-7. Epub 2013 Dec 19.
Lepista nuda (Bull. ex Fr.) Cooke belongs to Tricholomataceae and is an edible fungus with both economic and medical value. Mycelia were isolated from the fruiting bodies of L. nuda and were used to prepare the protoplast monokaryons. One hundred and fifteen monokaryons were obtained and their mating types were determined using somatic incompatibility tests. Protoplast monokaryons segregated into either the A1B1 or the A2B2 mating types. Inter-simple sequence repeats and sequence-related amplified polymorphism fingerprinting were used to analyse the mating types of these protoplast monokaryons and 16 sequence-characterised amplified region primers were developed to efficiently differentiate between the monokaryon mating types. Multiplex PCR analyses were also established. The data presented here outline a method for the precise and rapid identification of protoplast monokaryon mating types, which has the promise to shorten the period required for conventional crossbreeding.
光柄菇（Bull. ex Fr.）Cooke 属于伞菌科，是一种兼具经济和药用价值的食用真菌。本研究从光柄菇子实体中分离出菌丝体，用于制备原生质体单核体。获得了 115 个单核体，并通过体细胞不亲和性试验测定其交配型。原生质体单核体分离为 A1B1 或 A2B2 交配型。利用简单重复间序列和序列相关扩增多态性指纹图谱分析这些原生质体单核体的交配型，并开发了 16 个序列特征扩增区域引物，以有效地区分单核体交配型。还建立了多重 PCR 分析。本研究提供了一种精确、快速鉴定原生质体单核体交配型的方法，有望缩短常规杂交所需的时间。
