Yao Fang-Jie, Lu Li-Xin, Wang Peng, Fang Ming, Zhang You-Min, Chen Ying, Zhang Wei-Tong, Kong Xiang-Hui, Lu Jia, Honda Yoichi
College of Horticulture, Jilin Agricultural University, Changchun, Jilin Province, PR China.
Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, Jilin Province, PR China.
Mycobiology. 2018 Mar 29;46(1):72-78. doi: 10.1080/12298093.2018.1454004. eCollection 2018.
The fruiting body pattern is an important agronomic trait of the edible fungus , and an important breeding target. There are two types of fruiting body pattern: the cluster type and the chrysanthemum type. We identified the fruiting body pattern of 26 test strains, and then constructed two different near-isogenic pools. Then, we developed sequence characterized amplified region (SCAR) molecular markers associated with the fruiting body pattern based on sequence-related amplified polymorphism (SRAP) markers. Ten different bands (189-522 bp) were amplified using 153 pairs of SRAP primers. The SCAR marker "SCL-18" consisted of a single 522-bp band amplified from the cluster-type strains, but not the chrysanthemum strains. This SCAR marker was closely associated with the cluster-type fruiting body trait of . These results lay the foundation for further research to locate and clone genes controlling the fruiting body pattern of .
子实体形态是食用菌的一个重要农艺性状，也是一个重要的育种目标。子实体形态有两种类型：簇生型和菊花型。我们鉴定了26个测试菌株的子实体形态，然后构建了两个不同的近等基因池。接着，我们基于序列相关扩增多态性（SRAP）标记开发了与子实体形态相关的序列特征扩增区域（SCAR）分子标记。使用153对SRAP引物扩增出10条不同的条带（189 - 522 bp）。SCAR标记“SCL - 18”由从簇生型菌株中扩增出的一条单一的522 bp条带组成，而菊花型菌株中没有。这个SCAR标记与[具体食用菌名称]的簇生型子实体性状密切相关。这些结果为进一步定位和克隆控制[具体食用菌名称]子实体形态的基因奠定了基础。
