中国东北地区白褐鳞伞（伞菌目，担子菌门）的遗传多样性研究：基于 SRAP 和 ISSR 标记。
Genetic diversity of Lepista nuda (Agaricales, Basidiomycota) in Northeast China as indicated by SRAP and ISSR markers.
机构信息
College of Biological Science and Technology, Shenyang Agricultural University, Shenyang, Liaoning, China.
College of Life Engineering, Shenyang Institute of Technology, Fushun, Liaoning, China.
出版信息
PLoS One. 2018 Aug 27;13(8):e0202761. doi: 10.1371/journal.pone.0202761. eCollection 2018.
Lepista nuda is a popular wild edible mushroom that grows in China. In this study, we used ISSR and SRAP molecular markers to analyze the genetic diversity of 72 samples of L. nuda from eight populations in Northeast China. In total, six ISSR primers and five pairs of SRAP primers that produced clear and polymorphic banding profiles were selected for assessing L. nuda genetic diversity. The results revealed a high level of genetic variation among the 72 samples (94.4% polymorphism) but a low degree of gene flow among the populations. Among L. nuda populations, genetic distance was not correlated significantly with geographic distance. The antioxidant activity of the samples from each population was also tested and the result showed that all the selected samples had more than 60% DPPH scavenging activities. Nonetheless, the antioxidant activity diversity is not coincident with both the genetic diversity and the geographic distribution. The results indicate that ISSR and SRAP molecular markers are useful for studying the genetic diversity of L. nuda. The results also suggest that L. nuda populations in Northeast China require protection.
光秃鳞伞是一种在中国广泛分布的野生食用菌。本研究采用 ISSR 和 SRAP 分子标记技术，对来自中国东北地区 8 个种群的 72 个光秃鳞伞样本进行了遗传多样性分析。共筛选出 6 条 ISSR 引物和 5 对 SRAP 引物，这些引物产生了清晰且多态性的条带图谱，可用于评估光秃鳞伞的遗传多样性。结果表明，72 个样本具有较高的遗传变异水平（94.4%的多态性），但种群间的基因流较低。在光秃鳞伞种群中，遗传距离与地理距离之间没有显著相关性。还对每个种群的样本进行了抗氧化活性测试，结果表明所有选定的样本均具有超过 60%的 DPPH 清除活性。然而，抗氧化活性的多样性与遗传多样性和地理分布并不一致。研究结果表明，ISSR 和 SRAP 分子标记可用于研究光秃鳞伞的遗传多样性。同时，研究结果还提示中国东北地区的光秃鳞伞种群需要得到保护。
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