Nephrology Department, Myoung-Ji Hospital, Seoul I.
Kidney Blood Press Res. 2013;37(6):602-10. doi: 10.1159/000355740. Epub 2013 Dec 8.
BACKGROUND/AIM: Our aim was to determine whether altered human β-defensin (HBD), pro-inflammatory cytokines including interleukin (IL)-6 and tumor necrotic factor (TNF)-α could increase the risk of developing and exacerbation of chronic kidney disease (CKD), especially for patients with diabetic nephropathy (DN).
Serum samples were obtained from 338 CKD patients and 88 sex, age-matched healthy controls. The concentrations of HBD-1 were assayed using an RIA kit. Serum levels of HBD-2, IL-6 and TNF-α were assayed using an ELISA kit.
Serum levels of HBD-1, IL-6 and TNF-α were significantly higher in CKD patients compared to healthy controls (P<0.05). HBD-1 levels were inversely related to estimated glomerular filtration rate (eGFR), which was the coefficient factor (β = -0.357, P = 0.035) explaining the variability in HBD-1 in CKD. Diabetic nephropathy (DN) patients at stage 3-5 had significantly higher serum HBD-1 levels than non DN patients (P=0.00).
Our data support the view that there is increased inflammation in CKD and DN. The inverse correlation between eGFR and serum HBD-1 which we observed is suggestive of a relationship between innate immunity and renal function and should be further investigated.
背景/目的：我们的目的是确定人β防御素（HBD）的改变、包括白细胞介素（IL）-6 和肿瘤坏死因子（TNF）-α在内的促炎细胞因子是否会增加慢性肾脏病（CKD）的发生和恶化风险，尤其是对于糖尿病肾病（DN）患者。
从 338 例 CKD 患者和 88 名性别、年龄匹配的健康对照者中获得血清样本。使用 RIA 试剂盒测定 HBD-1 的浓度。使用 ELISA 试剂盒测定 HBD-2、IL-6 和 TNF-α的血清水平。
与健康对照组相比，CKD 患者的血清 HBD-1、IL-6 和 TNF-α水平显著升高（P<0.05）。HBD-1 水平与估算肾小球滤过率（eGFR）呈负相关，这是解释 CKD 中 HBD-1 变异性的系数因素（β=-0.357，P=0.035）。3-5 期的糖尿病肾病（DN）患者的血清 HBD-1 水平明显高于非 DN 患者（P=0.00）。
我们的数据支持 CKD 和 DN 中存在炎症增加的观点。我们观察到的 eGFR 与血清 HBD-1 之间的负相关表明先天免疫与肾功能之间存在关系，应进一步研究。
