Department of Life and Environmental Sciences, Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.
Department of Life and Environmental Sciences, Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.
Food Chem. 2014 May 1;150:477-81. doi: 10.1016/j.foodchem.2013.11.003. Epub 2013 Nov 12.
The study compares official spectrophotometric methods for the determination of proline content in honey - those of the International Honey Commission (IHC) and the Association of Official Analytical Chemists (AOAC) - with the original Ough method. Results show that the extra time-consuming treatment stages added by the IHC method with respect to the Ough method are pointless. We demonstrate that the AOACs method proves to be the best in terms of accuracy and time saving. The optimized waiting time for the absorbance recording is set at 35min from the removal of reaction tubes from the boiling bath used in the sample treatment. The optimized method was validated in the matrix: linearity up to 1800mgL(-1), limit of detection 20mgL(-1), limit of quantification 61mgL(-1). The method was applied to 43 unifloral honey samples from the Marche region, Italy.
该研究比较了官方分光光度法测定蜂蜜中天冬氨酸含量的方法，即国际蜂蜜委员会（IHC）和美国分析化学家协会（AOAC）的方法与 Ough 原始方法。结果表明，IHC 方法相对于 Ough 方法增加的额外耗时处理步骤是没有意义的。我们证明，就准确性和节省时间而言，AOAC 方法是最好的。记录吸光度的最佳等待时间设定为从用于样品处理的沸水中取出反应管后 35 分钟。在基质中验证了优化后的方法：线性度高达 1800mgL(-1)，检测限为 20mgL(-1)，定量限为 61mgL(-1)。该方法应用于来自意大利马尔凯地区的 43 种单花蜂蜜样品。
