Department of Chemistry, Indian Institute of Technology Delhi , New Delhi-110016, India.
Biomacromolecules. 2014 Feb 10;15(2):582-90. doi: 10.1021/bm4015655. Epub 2014 Jan 6.
We report on the self-assembly based fabrication of fibrous polymers for tissue engineering applications. Directed self-assembly followed by polymerization of lysine-appended diacetylenes generated a variety of polymers (P1-P5) with distinct chemical properties. The self-assembly along with the conjugated double and triple bonds and rigid geometry of diacetylene backbone imposed a nanofibrous morphology on the resulting polymers. Chemical properties including wettability of the polymers were tuned by using lysine (Lys) with orthogonal protecting groups (Boc and Fmoc). These Lys-appended polydiacetylene scaffolds were compared in terms of their efficiency toward human mesenchymal stem cells adhesion and spreading. Interestingly, polymer P4 containing Lys N(α)-NH2 and Lys N(ε)-Boc with balanced wettability supported cell adhesion better than the more hydrophobic polymer P2 with N(ε)-Boc and N(α)-Fmoc or more hydrophilic polymer P5 containing free N(ε) and N(α) amino groups. The molecular level control in the fabrication of nanofibrous polymers compared with other existing methods for the generation of fibrous polymers is the hallmark of this work.
我们报告了基于自组装的纤维状聚合物的制造，用于组织工程应用。赖氨酸修饰的二炔的定向自组装后聚合产生了具有不同化学性质的多种聚合物（P1-P5）。自组装以及共轭的双键和三键以及二炔骨架的刚性几何形状在所得聚合物上施加了纳米纤维形态。通过使用具有正交保护基团（Boc 和 Fmoc）的赖氨酸（Lys）来调整聚合物的化学性质，包括其润湿性。这些赖氨酸修饰的聚二炔支架在人骨髓间充质干细胞黏附和铺展方面的效率进行了比较。有趣的是，含有 Lys N(α)-NH2 和 Lys N(ε)-Boc 的聚合物 P4 具有平衡的润湿性，比具有 N(ε)-Boc 和 N(α)-Fmoc 的更疏水的聚合物 P2 或含有游离 N(ε)和 N(α)氨基的更亲水的聚合物 P5 更能支持细胞黏附。与其他用于生成纤维状聚合物的现有方法相比，这种在制造纳米纤维聚合物方面的分子水平控制是这项工作的特点。
