Department of Chemical and Materials Engineering, Tunghai University, Taichung 407, Taiwan.
Department of Chemical and Materials Engineering, Tunghai University, Taichung 407, Taiwan.
Bioresour Technol. 2014 Feb;153:374-8. doi: 10.1016/j.biortech.2013.12.005. Epub 2013 Dec 11.
The rapid accumulation of glycerol during biodiesel synthesis has attracted much interest on the potential usage. One of the promising applications is the conversion of glycerol to 1,3-propanediol (PDO), as well 2,3-butanediol (BDO), by using Klebsiella pneumonia. The results of this study indicate that the dissolved oxygen level (DO) is a determining factor in the distribution of PDO and BDO. The batch with a low DO could achieve a much higher PDO/BDO ratio than the high DO batch, with results of 9.9 and 0.2, respectively. The enzyme activity of glycerol dehydratase (GDHt) in the low DO batch was about three times that of the high DO batch, and this might be the cause of the enhanced PDO production. In conclusion, the results of this work show that high DO was beneficial to the production of BDO when using glycerol as the carbon source, while low DO could enhance PDO production.
在生物柴油合成过程中甘油的快速积累引起了人们对其潜在用途的极大兴趣。其中一个有前途的应用是利用肺炎克雷伯氏菌将甘油转化为 1,3-丙二醇（PDO）和 2,3-丁二醇（BDO）。本研究结果表明，溶解氧水平（DO）是影响 PDO 和 BDO 分布的决定因素。低 DO 批次比高 DO 批次能够实现更高的 PDO/BDO 比，分别为 9.9 和 0.2。低 DO 批次中的甘油脱水酶（GDHt）的酶活性约为高 DO 批次的三倍，这可能是增强 PDO 生产的原因。总之，这项工作的结果表明，当以甘油为碳源时，高 DO 有利于 BDO 的生产，而低 DO 可以提高 PDO 的产量。
