pH 值和发酵策略对一株分离的 Klebsiella sp. Zmd30 菌株生产 2,3-丁二醇的影响。
Effects of pH and fermentation strategies on 2,3-butanediol production with an isolated Klebsiella sp. Zmd30 strain.
机构信息
Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan.
Department of Chemical and Materials Engineering, Tunghai University, Taichung 407, Taiwan.
出版信息
Bioresour Technol. 2014;152:169-76. doi: 10.1016/j.biortech.2013.10.101. Epub 2013 Nov 5.
This study examined the effects of pH-controlled and fermentation strategies on 2,3-butanediol (2,3-BDO) production by an isolated Klebsiella sp. Zmd30 strain. The pH value of 6.0 was found to be optimal among the investigated range of 4.5-9.0. In batch fermentation, the concentration, productivity and yield of 2,3-BDO were 57.17 g/l, 1.59 g/l/h and 82%, respectively. The 2,3-BDO production by Klebsiella sp. Zmd30 was found to be growth-associated. Higher 2,3-BDO concentration (110 g/l) and yield (94%) was obtained by using fed-batch operation, but the productivity was lower (0.88 g/l/h) as compared to that when using batch operation. The highest 2,3-BDO productivity of 2.81 g/l/h was obtained with the continuous culture at a hydraulic retention time (HRT) of 12h. The results suggest that fed-batch operation might be most suitable for commercialized 2,3-BDO production, due to obtaining a high concentration and yield.
本研究考察了 pH 控制和发酵策略对一株分离的克氏杆菌 Zmd30 菌株生产 2,3-丁二醇（2,3-BDO）的影响。在研究的 4.5-9.0 范围内，发现 pH 值 6.0 是最佳的。在分批发酵中，2,3-BDO 的浓度、生产率和产率分别为 57.17 g/l、1.59 g/l/h 和 82%。发现克氏杆菌 Zmd30 的 2,3-BDO 生产与生长相关。通过使用补料分批操作，获得了更高的 2,3-BDO 浓度（110 g/l）和产率（94%），但生产率（0.88 g/l/h）比分批操作低。在水力停留时间（HRT）为 12h 的连续培养中，获得了最高的 2,3-BDO 生产率 2.81 g/l/h。结果表明，由于获得了较高的浓度和产率，补料分批操作可能最适合商业化的 2,3-BDO 生产。
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