Yen Hong-Wei, Li Fang-Tzu, Wong Chiao-Ling, Chang Jo-Shu
Department of Chemical and Materials Engineering, Tunghai University, Taichung, 407, Taiwan,
Bioprocess Biosyst Eng. 2014 Mar;37(3):425-31. doi: 10.1007/s00449-013-1008-1. Epub 2013 Jul 14.
Several carbon sources were investigated for the production of 1,3-propanediol (PDO) and 2,3-butanediol (BDO) simultaneously, using an isolated indigenous Klebsiella sp. Ana-WS5. The results indicate that glycerol is a suitable carbon source for both BDO and PDO production. Further investigation suggests that adjustment of the pH could alter the metabolic pathway, which affects the ratio of PDO and BDO obtained. The batch with pH controlled at 7.0 had the highest total diol (PDO + BDO) productivity of 0.86 g/L h and the highest PDO/BDO of 7.67, as compared to a batch with pH controlled at 6.0. However, the batch without pH control could achieve a maximum total diol concentration of 48.1 ± 1.6 g/L and the highest yield of 86 % (total diols produced/glycerol consumed). The effects of pH control on the distribution of PDO and BDO concluded in this study could be further applied to the process design for enhancing PDO or BDO production.
使用分离出的本地克雷伯氏菌Ana-WS5，研究了几种碳源用于同时生产1,3-丙二醇（PDO）和2,3-丁二醇（BDO）的情况。结果表明，甘油是生产BDO和PDO的合适碳源。进一步研究表明，调节pH值可以改变代谢途径，从而影响获得的PDO和BDO的比例。与pH值控制在6.0的批次相比，pH值控制在7.0的批次总二醇（PDO + BDO）生产率最高，为0.86 g/L·h，PDO/BDO最高，为7.67。然而，未控制pH值的批次可实现最大总二醇浓度为48.1±1.6 g/L，最高产率为86%（总二醇产量/消耗的甘油）。本研究得出的pH值控制对PDO和BDO分布的影响可进一步应用于提高PDO或BDO产量的工艺设计。
