Mushroom Research Centre, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Mushroom Research Centre, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Evid Based Complement Alternat Med. 2013;2013:304713. doi: 10.1155/2013/304713. Epub 2013 Nov 25.
Amauroderma rugosum is a wild mushroom that is worn as a necklace by the indigenous communities in Malaysia to prevent fits and incessant crying by babies. The aim of this study was to investigate the nutritive composition and antioxidant potential and anti-inflammatory effects of A. rugosum extracts on LPS-stimulated RAW264.7 cells. Nutritional analysis of freeze-dried mycelia of A. rugosum (KUM 61131) from submerged culture indicated a predominant presence of carbohydrates, proteins, dietary fibre, phosphorus, potassium, and sodium. The ethanol crude extract (EE), its hexane (HF), ethyl acetate (EAF), and aqueous (AF) fractions of mycelia of A. rugosum grown in submerged culture were evaluated for antioxidant potential and anti-inflammatory effects. EAF exhibited the highest total phenolic content and the strongest antioxidant activity based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays. HF showed dose-dependent inhibition of NO production in LPS-stimulated RAW264.7 cells and NO radical scavenging activity. Gas chromatographic analysis of HF revealed the presence of ethyl linoleate and ergosterol, compounds with known anti-inflammatory properties. In conclusion, the nutritive compositions and significant antioxidant potential and anti-inflammatory effects of mycelia extracts of A. rugosum have the potential to serve as a therapeutic agent or adjuvant in the management of inflammatory disorders.
皱红冬孢酵母是一种野生蘑菇，马来西亚的土著社区将其佩戴在项链上，以防止婴儿发作和持续哭泣。本研究旨在研究皱红冬孢酵母（KUM 61131）浸出培养物的菌丝体的营养成分、抗氧化潜力和对 LPS 刺激的 RAW264.7 细胞的抗炎作用。皱红冬孢酵母（KUM 61131）浸出培养物菌丝体的冷冻干燥物的营养分析表明，碳水化合物、蛋白质、膳食纤维、磷、钾和钠的含量较高。评估了皱红冬孢酵母（KUM 61131）浸出培养物菌丝体的乙醇粗提取物（EE）、其正己烷（HF）、乙酸乙酯（EAF）和水（AF）馏分的抗氧化潜力和抗炎作用。EAF 表现出最高的总酚含量和最强的抗氧化活性，基于 2,2-二苯基-1-苦基肼（DPPH）和 2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）测定法。HF 对 LPS 刺激的 RAW264.7 细胞中 NO 产生具有剂量依赖性抑制作用，且具有 NO 自由基清除活性。HF 的气相色谱分析显示存在具有已知抗炎特性的亚油酸乙酯和麦角固醇。综上所述，皱红冬孢酵母菌丝体提取物的营养成分、显著的抗氧化潜力和抗炎作用，有可能作为炎症性疾病治疗剂或佐剂。
