Zhang Lin, Khoo Cheang Soo, Koyyalamudi Sundar Rao, Reddy Narsimha
Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, 100010, China.
Beijing Institute of Chinese Medicine, Beijing, 100010, China.
Heliyon. 2024 May 22;10(11):e31672. doi: 10.1016/j.heliyon.2024.e31672. eCollection 2024 Jun 15.
(Blume and T. Nees) Torrend is a traditionally well-known mushroom that is used for the treatment of cancer. In order to evaluate the pharmacological activities of polysaccharides, the mushroom powder was subjected to hot water extraction and pure polysaccharides (ARPs) were isolated by gel-filtration method. Three important APRs called ARP-1, ARP-2 and ARP-5 were identified with average molecular weights of 1494, 450, and 7 kDa respectively. Their antioxidant abilities were estimated by examining free radical scavenging potential against 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid radical (ABTS), 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), and hydroxyl radical. Immunomodulatory potentials of these ARPs were determined using murine macrophage cells. These polysaccharides exhibited high antioxidant abilities and stimulated mouse macrophages leading to the generation of tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6). Excellent activities were displayed by ARP-1 and APR-2. Gas chromatography and spectroscopic (FT-IR and NMR) methods were employed in order to carry out their structural characterisation. The two high molecular weight ARPs (ARP-1 and ARP-2) displayed β-(1 → 3)-D-glucan backbone structure with branching of β-(1 → 6)-d-glucopyranosyl. These observations suggest high potential of ARPs for immunotherapeutic applications.
（布卢姆和T. 内斯）托伦德是一种传统上广为人知的蘑菇，用于治疗癌症。为了评估多糖的药理活性，对蘑菇粉进行热水提取，并通过凝胶过滤法分离出纯多糖（ARPs）。鉴定出三种重要的ARPs，分别称为ARP - 1、ARP - 2和ARP - 5，平均分子量分别为1494、450和7 kDa。通过检测对2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）自由基（ABTS）、2,2-二苯基-1-苦基肼自由基（DPPH）和羟基自由基的自由基清除潜力来评估它们的抗氧化能力。使用小鼠巨噬细胞确定这些ARPs的免疫调节潜力。这些多糖表现出高抗氧化能力，并刺激小鼠巨噬细胞产生肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）。ARP - 1和ARP - 2表现出优异的活性。采用气相色谱和光谱（傅里叶变换红外光谱和核磁共振）方法对它们进行结构表征。两种高分子量的ARPs（ARP - 1和ARP - 2）显示出具有β-(1→3)-D-葡聚糖主链结构，并带有β-(1→6)-D-吡喃葡萄糖基分支。这些观察结果表明ARPs在免疫治疗应用方面具有很高的潜力。
