芹菜素具有抗萎缩性胃炎和抗幽门螺杆菌感染蒙古沙土鼠胃癌进展的作用。

Apigenin has anti-atrophic gastritis and anti-gastric cancer progression effects in Helicobacter pylori-infected Mongolian gerbils.

机构信息

Division of Gastroenterology, Department of Internal Medicine and Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan, ROC; Department of Medicine, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC.

Division of Gastroenterology, Department of Internal Medicine and Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan, ROC; Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan, ROC.

出版信息

J Ethnopharmacol. 2014 Feb 12;151(3):1031-1039. doi: 10.1016/j.jep.2013.11.040. Epub 2013 Dec 25.

DOI:10.1016/j.jep.2013.11.040

PMID:24374236

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Apigenin, one of the most common flavonoids, is abundant in celery, parsley, chamomile, passionflower, and other vegetables and fruits. Celery is recognized as a medicinal vegetable in Oriental countries to traditionally treat inflammation, swelling, blood pressure, serum lipid, and toothache. In this study, we investigated apigenin treatment effects on Helicobacter pylori-induced atrophic gastritis and gastric cancer progression in Mongolian gerbils.

MATERIALS AND METHODS

Five to eight-week-old Mongolian gerbils were inoculated with Helicobacter pylori for four weeks without (atrophic gastritis group) or with N'-methyl-N'-nitro-N-nitroso-guanidine (MNNG) (gastric cancer group) in drinking water, and were then rested for two weeks. During the 7th-32th (atrophic gastritis group) or the 7th-52th (gastric cancer group) weeks, they were given various doses (0-60 mg/kgbw/day) of apigenin. At the end of the 32th (atrophic gastritis group) or the 52th (atrophic gastritis group) week, all Mongolian gerbils were sacrificed using the CO2 asphyxia method. The histological changes of Helicobacter pylori colonization, neutrophil and monocyte infiltrations, and atrophic gastritis in both atrophic gastritis and gastric cancer Mongolian gerbils were examined using immunohistochemistry stain and Sydney System scoring.

RESULTS

Apigenin treatments (30-60 mg/kgbw/day) effectively decreased atrophic gastritis (atrophic gastritis group) and dysplasia/gastric cancer (gastric cancer group) rates in Mongolian gerbils. Apigenin treatment (60 mg/kgbw/day) significantly decreased Helicobacter pylori colonization and Helicobacter pylori-induced histological changes of neutrophil and monocyte infiltrations and atrophic gastritis in both atrophic gastritis and gastric cancer Mongolian gerbils.

CONCLUSIONS

Apigenin has the remarkable ability to inhibit Helicobacter pylori-induced atrophic gastritis and gastric cancer progression as well as possessing potent anti-gastric cancer activity.

摘要

植物药关联性

芹菜、欧芹、洋甘菊、西番莲等蔬菜和水果中富含芹菜素，它是最常见的类黄酮之一。芹菜在东方国家被视为一种药用蔬菜，传统上用于治疗炎症、肿胀、血压、血脂和牙痛。在这项研究中，我们研究了芹菜素对蒙古沙土鼠幽门螺杆菌诱导的萎缩性胃炎和胃癌进展的治疗作用。

方法

5 至 8 周龄蒙古沙土鼠经口接种幽门螺杆菌 4 周（萎缩性胃炎组）或在饮用水中添加 N′-甲基-N′-硝基-N-亚硝基胍（MNNG）（胃癌组），然后休息 2 周。在第 7 至 32 周（萎缩性胃炎组）或第 7 至 52 周（胃癌组）期间，给予不同剂量（0-60mg/kgbw/天）的芹菜素。在第 32 周（萎缩性胃炎组）或第 52 周（胃癌组）结束时，所有蒙古沙土鼠均采用 CO2 窒息法处死。采用免疫组化染色和悉尼系统评分法检测蒙古沙土鼠幽门螺杆菌定植、中性粒细胞和单核细胞浸润以及萎缩性胃炎的组织学变化。