R&D Center, Somang Cosmetics Corporation, 687-14 Kozan-dong, Namdong-gu, Incheon, 405-310, Republic of Korea.
Phytother Res. 2014 Jul;28(7):1088-95. doi: 10.1002/ptr.5101. Epub 2013 Dec 26.
Crude Panax ginseng has been documented to possess hair growth activity and is widely used to treat alopecia, but the effects of ginsenoside Rg3 on hair growth have not to our knowledge been determined. The aim of the current study was to identify the molecules through which Rg3 stimulates hair growth. The thymidine incorporation for measuring cell proliferation was determined. We used DNA microarray analysis to measure gene expression levels in dermal papilla (DP) cells upon treatment with Rg3. The mRNA and protein expression levels of vascular endothelial growth factor (VEGF) in human DP cells were measured by real-time polymerase chain reaction and immunohistochemistry, respectively. We also used immunohistochemistry assays to detect in vivo changes in VEGF and 3-stemness marker expressions in mouse hair follicles. Reverse transcription polymerase chain reaction showed dose-dependent increases in VEGF mRNA levels on treatment with Rg3. Immunohistochemical analysis showed that expression of VEGF was significantly up-regulated by Rg3 in a dose-dependent manner in human DP cells and in mouse hair follicles. In addition, the CD8 and CD34 were also up-regulated by Rg3 in the mouse hair follicles. It may be concluded that Rg3 might increase hair growth through stimulation of hair follicle stem cells and it has the potential to be used in hair growth products.
生晒参已被证明具有生发活性，被广泛用于治疗脱发，但迄今为止，我们尚未了解人参皂苷 Rg3 对毛发生长的影响。本研究旨在确定 Rg3 刺激毛发生长的分子机制。通过噻唑蓝（MTT）比色法检测细胞增殖。我们使用 DNA 微阵列分析来测量 Rg3 处理后真皮乳头（DP）细胞的基因表达水平。通过实时聚合酶链反应和免疫组织化学分别测量人 DP 细胞中血管内皮生长因子（VEGF）的 mRNA 和蛋白表达水平。我们还使用免疫组织化学检测 VEGF 和 3 干性标志物在小鼠毛囊中的体内变化。逆转录聚合酶链反应显示 Rg3 处理后 VEGF mRNA 水平呈剂量依赖性增加。免疫组织化学分析显示，Rg3 以剂量依赖性方式显著上调人 DP 细胞和小鼠毛囊中 VEGF 的表达。此外，Rg3 还上调了小鼠毛囊中的 CD8 和 CD34。因此，Rg3 可能通过刺激毛囊干细胞来增加毛发生长，它有可能被用于生发产品。
