UV-C 巴氏消毒对供体人乳抑菌特性和免疫蛋白的影响。
The effect of UV-C pasteurization on bacteriostatic properties and immunological proteins of donor human milk.
机构信息
School of Chemistry and Biochemistry, Faculty of Science, The University of Western Australia, Crawley, Western Australia, Australia ; Carag AG, Baar, Switzerland.
School of Chemistry and Biochemistry, Faculty of Science, The University of Western Australia, Crawley, Western Australia, Australia.
出版信息
PLoS One. 2013 Dec 23;8(12):e85867. doi: 10.1371/journal.pone.0085867. eCollection 2013.
BACKGROUND
Human milk possesses bacteriostatic properties, largely due to the presence of immunological proteins. Heat treatments such as Holder pasteurization reduce the concentration of immunological proteins in human milk and consequently increase the bacterial growth rate. This study investigated the bacterial growth rate and the immunological protein concentration of ultraviolet (UV-C) irradiated, Holder pasteurized and untreated human milk.
METHODS
Samples (n=10) of untreated, Holder pasteurized and UV-C irradiated human milk were inoculated with E. coli and S. aureus and the growth rate over 2 hours incubation time at 37°C was observed. Additionally, the concentration of sIgA, lactoferrin and lysozyme of untreated and treated human milk was analyzed.
RESULTS
The bacterial growth rate of untreated and UV-C irradiated human milk was not significantly different. The bacterial growth rate of Holder pasteurized human milk was double compared to untreated human milk (p<0.001). The retention of sIgA, lactoferrin and lysozyme after UV-C irradiation was 89%, 87%, and 75% respectively, which were higher than Holder treated with 49%, 9%, and 41% respectively.
CONCLUSION
UV-C irradiation of human milk preserves significantly higher levels of immunological proteins than Holder pasteurization, resulting in bacteriostatic properties similar to those of untreated human milk.
背景
人乳具有抑菌特性，这主要归因于免疫蛋白的存在。热处理（如 Holder 巴氏消毒法）会降低人乳中免疫蛋白的浓度，从而加快细菌的生长速度。本研究旨在比较紫外线（UV-C）照射、Holder 巴氏消毒法和未处理的人乳的细菌生长速度和免疫蛋白浓度。
方法
将未经处理、Holder 巴氏消毒和 UV-C 照射的人乳样本（n=10）接种大肠杆菌和金黄色葡萄球菌，在 37°C 孵育 2 小时，观察细菌生长速度。此外，还分析了未经处理和处理后的人乳中 sIgA、乳铁蛋白和溶菌酶的浓度。
结果
未经处理和 UV-C 照射的人乳的细菌生长速度无显著差异。Holder 巴氏消毒的人乳的细菌生长速度是未经处理人乳的两倍（p<0.001）。UV-C 照射后人乳中 sIgA、乳铁蛋白和溶菌酶的保留率分别为 89%、87%和 75%，均高于 Holder 巴氏消毒的 49%、9%和 41%。
结论
与 Holder 巴氏消毒相比，UV-C 照射能显著保留更多的免疫蛋白，从而为人乳提供类似未经处理的人乳的抑菌特性。
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