Yun Yu-Xia, Wang Yan-Ping, Wang Peng, Cui Li-Hong, Wang Kai-Juan, Zhang Jian-Ying, Dai Li-Ping
Department of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University, Zhengzhou, China E-mail :
Asian Pac J Cancer Prev. 2014 Jan;14(11):6507-12. doi: 10.7314/apjcp.2013.14.11.6507.
The purpose of this study was to evaluate the associations of CYP1A1 genetic polymorphisms with the risk of developing esophageal cancer (EC). A case-control study was carried out in a Chinese population in which 157 hospital based EC cases and 157 population based healthy controls with 1:1 match by age and sex were included. PCR based restriction fragment length polymorphisms (PCR-RFLP) were used to detect genotypes in case and control groups. For the CYP1A1 Ile/Val polymorphism, comparing with wild genotype Ile/Ile, both the heterozygote genotype Ile/Val and the combined variant genotype Ile/Val+Val/Val increased the risk of esophageal cancer (OR: 2.05, 95%CI: 1.19-3.54, OR: 1.86, 95%CI: 1.11-3.12). No significant association was found between the CYP1A1 MspI polymorphism and EC. According to analysis of combined genotypes, the TC/AG combined genotype which contained both variant alleles of these two polymorphisms increased the risk of developing EC (OR: 2.12, 95%CI: 1.16-3.85). Our results suggested that genetic polymorphisms of CYP1A1 may increase the susceptibility to EC.
本研究的目的是评估CYP1A1基因多态性与食管癌（EC）发生风险之间的关联。在中国人群中开展了一项病例对照研究，纳入了157例基于医院的EC病例和157例基于人群的健康对照，病例与对照按年龄和性别1:1匹配。采用基于聚合酶链反应（PCR）的限制性片段长度多态性分析（PCR-RFLP）检测病例组和对照组的基因型。对于CYP1A1 Ile/Val多态性，与野生基因型Ile/Ile相比，杂合子基因型Ile/Val和组合变异基因型Ile/Val+Val/Val均增加了食管癌风险（比值比：2.05，95%置信区间：1.19 - 3.54；比值比：1.86，95%置信区间：1.11 - 3.12）。未发现CYP1A1 MspI多态性与EC之间存在显著关联。根据组合基因型分析，包含这两种多态性变异等位基因的TC/AG组合基因型增加了发生EC的风险（比值比：2.12，95%置信区间：1.16 - 3.85）。我们的结果表明，CYP1A1基因多态性可能增加对EC的易感性。
