Li D, Peng J J, Tan Y, Chen T, Wei D, Du M, Zhang T
Department of Oncology, Chengdu Military General Hospital, Chengdu, Sichuan Province, China.
Department of Oncology, Chengdu Military General Hospital, Chengdu, Sichuan Province, China
Genet Mol Res. 2015 Mar 20;14(1):1926-31. doi: 10.4238/2015.March.20.2.
We conducted a hospital-based case-control study to investigate the effect of the miR-146aG>C and miR-499A>G polymorphisms on the risk of hepatocellular carcinoma (HCC) in a Chinese population. This study was 1:1 matched case-control study consisting of 184 HCC patients and 184 control subjects. miR-146aG>C and miR-499A>G polymorphisms were genotyped by polymerase chain reaction-restriction fragment length polymorphism. Multivariate regression analyses showed that subjects carrying the miR-146a G allele and miR-499 G allele were associated with a non-significant increased risk of HCC compared with subjects with the homozygous allele, with adjusted odds ratios (95% confidence interval) of 1.38 (0.97-1.84) and 1.40 (0.99-2.08), respectively. Moreover, subjects carrying the miR-499 A allele showed a greatly increased risk of HCC in subjects infected with HBV compared with subjects carrying the miR-499 A allele, with an adjusted odds ratio (95% confidence interval) of 1.53 (1.34-2.41). In conclusion, the miR-146aG>C and miR-499A>G polymorphisms do not have a role in the genetic susceptibility to HCC.
我们开展了一项基于医院的病例对照研究，以调查miR-146aG>C和miR-499A>G多态性对中国人群肝细胞癌（HCC）风险的影响。本研究为1:1匹配的病例对照研究，包括184例HCC患者和184例对照受试者。采用聚合酶链反应-限制性片段长度多态性方法对miR-146aG>C和miR-499A>G多态性进行基因分型。多因素回归分析显示，与纯合子等位基因受试者相比，携带miR-146a G等位基因和miR-499 G等位基因的受试者患HCC的风险虽有增加但无统计学意义，调整后的比值比（95%置信区间）分别为1.38（0.97-1.84）和1.40（0.99-2.08）。此外，与携带miR-499 A等位基因的受试者相比，携带miR-499 A等位基因的HBV感染者患HCC的风险大幅增加，调整后的比值比（95%置信区间）为1.53（1.34-2.41）。总之，miR-146aG>C和miR-499A>G多态性在HCC的遗传易感性中不起作用。
