Department of Physics, Chemistry and Pharmacy, MEMPHYS-Center for Biomembrane Physics, University of Southern Denmark , Campusvej 55, 5230 Odense M, Denmark.
Langmuir. 2014 Jan 14;30(1):13-6. doi: 10.1021/la403565f. Epub 2014 Jan 3.
In biophysical and biochemical studies of lipid bilayers the influence of the used buffer is often ignored or assumed to be negligible on membrane structure, elasticity, or physical properties. However, we here present experimental evidence, through bending rigidity measurements performed on giant vesicles, of a more complex behavior, where the buffering molecules may considerably affect the bending rigidity of phosphatidylcholine bilayers. Furthermore, a synergistic effect on the bending modulus is observed in the presence of both salt and buffer molecules, which serves as a warning to experimentalists in the data interpretation of their studies, since typical lipid bilayer studies contain buffer and ion molecules.
在脂质双层的生物物理和生化研究中，通常忽略或假设所使用的缓冲液对膜结构、弹性或物理性质的影响可以忽略不计。然而，我们在这里通过在巨大囊泡上进行弯曲刚性测量提供了实验证据，表明存在更复杂的行为，其中缓冲分子可能会极大地影响磷脂双层的弯曲刚性。此外，在盐和缓冲分子存在的情况下观察到弯曲模量的协同效应，这为实验人员在解释其研究数据时提供了警示，因为典型的脂质双层研究包含缓冲液和离子分子。
