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大蒜素的抗菌活性及其对 U2OS 人骨肉瘤细胞的作用。

Antibacterial Activity of Elephant Garlic and Its Effect against U2OS Human Osteosarcoma Cells.

机构信息

Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing 100083, China.

出版信息

Iran J Basic Med Sci. 2013 Oct;16(10):1088-94.

PMID:24379966
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3874095/
Abstract

OBJECTIVE(S): The present study was designed to investigate the antibacterial function and pharmacological effect of elephant garlic (Allium ampeloprasum var. ampeloprasum) on U2OS human osteosarcoma cells.

MATERIALS AND METHODS

Seven kinds of bacteria were reconstituted, inoculated and tested in this research to evaluate elephant garlic antibacterial activity. By the means of FACS analysis, cell proliferation assay, confocal laser scanning microscopy and Transwell migration assays, the effect of elephant garlic against U2OS human osteosarcoma cells was unveiled. Rerults: The antimicrobial activity of elephant garlic was stronger than ampicillin when used against Escherichia coli, Bacillus subtilis, Bacillus thuringiensis, Staphylococcus actinomycetes, and gray actinomycetes. Even at a very low concentration (12.5%), elephant garlic still had an antibacterial effect on common bacteria E. coli and S. aureus. The G0/G1 ratio of elephant garlic treated group cells increased while S phase decreased. Elephant garlic extract inhibited the growth of human osteosarcoma cells, U2OS, through preventing the transition from G1 phase to S phase. It reduced osteosarcoma cell, U2OS, invasion ability and significantly increased the proportion of apoptosis. It significantly affected the cytoskeleton generation.

CONCLUSION

Elephant garlic exhibits antibacterial property and has an inhibitory effect on osteosarcoma cells (U2OS) proliferation and cell activity, suggesting the mechanism of its anticancer effects on U2OS human osteosarcoma cells.

摘要

目的

本研究旨在探讨大蒜（Allium ampeloprasum var. ampeloprasum）对 U2OS 人骨肉瘤细胞的抗菌功能和药理作用。

材料和方法

本研究中重新配制、接种和测试了 7 种细菌，以评估大蒜的抗菌活性。通过流式细胞术分析、细胞增殖试验、共聚焦激光扫描显微镜和 Transwell 迁移试验，揭示了大蒜对 U2OS 人骨肉瘤细胞的作用。结果：与氨苄西林相比，大蒜对大肠杆菌、枯草芽孢杆菌、苏云金芽孢杆菌、放线菌 actinomycetes 和灰色放线菌的抗菌活性更强。即使在非常低的浓度（12.5%）下，大蒜对常见细菌大肠杆菌和金黄色葡萄球菌仍具有抗菌作用。大蒜处理组细胞的 G0/G1 比值增加，而 S 期减少。大蒜提取物通过阻止 G1 期向 S 期的转化，抑制人骨肉瘤细胞 U2OS 的生长。它降低了骨肉瘤细胞 U2OS 的侵袭能力，并显著增加了细胞凋亡的比例。它显著影响了细胞骨架的生成。

结论

大蒜具有抗菌特性，并对骨肉瘤细胞（U2OS）的增殖和细胞活性具有抑制作用，提示其对 U2OS 人骨肉瘤细胞的抗癌作用机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1382/3874095/5d74e111f514/ijbms-16-1088-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1382/3874095/970767dc1ba3/ijbms-16-1088-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1382/3874095/1cde7e2ecb1c/ijbms-16-1088-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1382/3874095/d19d74cf926e/ijbms-16-1088-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1382/3874095/16c601f0be71/ijbms-16-1088-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1382/3874095/14cbad1790f8/ijbms-16-1088-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1382/3874095/5d74e111f514/ijbms-16-1088-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1382/3874095/970767dc1ba3/ijbms-16-1088-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1382/3874095/1cde7e2ecb1c/ijbms-16-1088-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1382/3874095/d19d74cf926e/ijbms-16-1088-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1382/3874095/16c601f0be71/ijbms-16-1088-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1382/3874095/14cbad1790f8/ijbms-16-1088-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1382/3874095/5d74e111f514/ijbms-16-1088-g006.jpg

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