Wang Y L, Goren M B
J Cell Biol. 1987 Jun;104(6):1749-54. doi: 10.1083/jcb.104.6.1749.
It has previously been inferred that the fusion of a macrophage secondary lysosome with a phagosome delivers the entire lysosomal contents uniformly to the phagosome. We found, however, that different fluorescent lysosomal probes can enter phagosomes at remarkably different rates, even though they are initially sequestered together in the same organelles. Thus, sulforhodamine is almost exclusively delivered to yeast-containing phagosomes within 2 h of phagocytosis. But fluoresceinated, high molecular weight dextran accumulates in the same phagosomes only over a period of approximately 24 h. We postulate that the delivery of lysosomal contents may involve an intermittent and incremental process in which individual components can be selectively and sequentially transferred.
先前曾推断，巨噬细胞次级溶酶体与吞噬体的融合会将整个溶酶体内容物均匀地传递至吞噬体。然而，我们发现，不同的荧光溶酶体探针进入吞噬体的速率显著不同，即便它们最初一同被隔离在相同的细胞器中。因此，在吞噬作用发生后的2小时内，磺基罗丹明几乎完全被传递至含有酵母的吞噬体中。但荧光素标记的高分子量葡聚糖仅在大约24小时的时间段内积累于相同的吞噬体中。我们推测，溶酶体内容物的传递可能涉及一个间歇性的渐进过程，其中各个成分能够被选择性地、依次地转移。
