CDC42 在宫颈鳞癌中的表达及其与临床病理特征的相关性。
Expression of CDC42 in cervical squamous cell carcinoma and its correlation with clinicopathologic characteristics.
机构信息
Department of Gynecology and Obstetrics, Peking University Third Hospital, Beijing 100191, China;
Institute of Vascular Medicine, Peking University Third Hospital and Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptide, Ministry of Health, Beijing 100191, China.
出版信息
Chin J Cancer Res. 2013 Dec;25(6):656-61. doi: 10.3978/j.issn.1000-9604.2013.11.04.
OBJECTIVE
The high expression of cell division cycle 42 protein (CDC42) may be involved in the occurrence and progression of several tumors. However, the expression and function of CDC42 in cervical squamous cell carcinoma remains unclear. This study aimed to investigate the expression of CDC42 in cervical squamous cell carcinoma and its correlation with clinicopathologic characteristics.
METHODS
The expression of CDC42 in 162 cervical squamous cell carcinoma tissue samples and 33 normal cervical tissue samples was investigated by immunohistochemistry. The CDC42 mRNA expression was detected by reverse transcription-polymerase chain reaction (RT-PCR).
RESULTS
The cervical squamous cell carcinoma group showed a significantly higher CDC42 positive rate, compared to the normal cervical tissues (P<0.05). Furthermore, the tissues of stage II-IV carcinoma patients showed higher CDC42 expression levels compared to stage I patients (P=0.05). In addition, the expression of CDC42 was not correlated to age of patients, differentiation degree of cancer cells, or lymph node metastasis (P>0.05). Furthermore, compare with normal cervical tissues, the CDC42 mRNA expression in cervical cancer had no significant difference.
CONCLUSIONS
CDC42 was up-regulated at protein level, but not mRNA level, in cervical squamous cell carcinoma. The high expression of CDC42 was correlated to the clinical stage of the patients, indicating that CDC42 might contribute to the progression of cervical squamous cell carcinoma.
目的
细胞分裂周期蛋白 42（CDC42）的高表达可能与多种肿瘤的发生和发展有关。然而，CDC42 在宫颈鳞状细胞癌中的表达和功能仍不清楚。本研究旨在探讨 CDC42 在宫颈鳞状细胞癌中的表达及其与临床病理特征的关系。
方法
采用免疫组织化学法检测 162 例宫颈鳞状细胞癌组织标本和 33 例正常宫颈组织标本中 CDC42 的表达，采用逆转录-聚合酶链反应（RT-PCR）检测 CDC42 mRNA 的表达。
结果
宫颈鳞状细胞癌组 CDC42 阳性率明显高于正常宫颈组织（P<0.05）。此外，Ⅱ～Ⅳ期癌组织中 CDC42 的表达水平高于Ⅰ期患者（P=0.05）。此外，CDC42 的表达与患者年龄、癌细胞分化程度或淋巴结转移无关（P>0.05）。此外，与正常宫颈组织相比，宫颈癌中 CDC42 mRNA 的表达无显著差异。
结论
CDC42 在宫颈鳞状细胞癌中蛋白水平上调，而 mRNA 水平无明显变化。CDC42 的高表达与患者的临床分期相关，提示 CDC42 可能参与宫颈鳞状细胞癌的进展。
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