氧四环素、普罗帕酮和甲灭酸对 A549 或 Hela 细胞的选择性杀伤作用。
Selective killing effect of oxytetracycline, propafenone and metamizole on A549 or Hela cells.
机构信息
Department of Histology and Embryology, School of Medicine, Hubei University of Art and Science, Xiangyang 441053, China.
出版信息
Chin J Cancer Res. 2013 Dec;25(6):662-70. doi: 10.3978/j.issn.1000-9604.2013.11.05.
Abstract
OBJECTIVE
To determine the selective killing effect of oxytetracycline, propafenone and metamizole on A549 or Hela cells.
METHODS
Proliferation assay, lactate dehydrogenase (LDH) assay, apoptosis detecting, flow cytometry and western blot were performed.
RESULTS
It was found that treatment with propafenone at the concentration of 0.014 g/L or higher for 48 h could induce apoptosis in Hela cells greatly, while it was not observed in oxytetracycline and metamizole at the concentration of 0.20 g/L for 48 h. Oxytetracycline, propafenone and metamizole all displayed evident inhibitory effects on the proliferation of A549 cells. The results of LDH assay demonstrated that the drugs at the test range of concentration did not cause necrosis in the cells. Propafenone could elevate the protein level of P53 effectively (P<0.01).
CONCLUSIONS
Oxytetracycline, propafenone and metamizol (dipyrone) all displayed evident inhibitory effects on the proliferation of A549 cells. Propafenone also displayed evident inhibitory effects on the proliferation of Hela cells.
摘要
目的
研究土霉素、普罗帕酮和氨基比林（安乃近）对 A549 或 Hela 细胞的选择性杀伤作用。
方法
采用细胞增殖实验、乳酸脱氢酶（LDH）释放实验、流式细胞术和 Western blot 等方法进行研究。
结果
研究发现，普罗帕酮浓度为 0.014 g/L 或更高时，处理 48 h 可明显诱导 Hela 细胞发生凋亡，而土霉素和氨基比林浓度为 0.20 g/L 时，48 h 未观察到细胞凋亡。土霉素、普罗帕酮和氨基比林均对 A549 细胞的增殖有明显的抑制作用。LDH 实验结果表明，在所测试浓度范围内，药物未引起细胞坏死。普罗帕酮可有效提高 P53 蛋白水平（P<0.01）。
结论
土霉素、普罗帕酮和氨基比林（安乃近）均对 A549 细胞的增殖有明显的抑制作用，普罗帕酮对 Hela 细胞的增殖也有明显的抑制作用。
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