Costa Michael A, Marques Joaquim V, Dalisay Doralyn S, Herman Barrington, Bedgar Diana L, Davin Laurence B, Lewis Norman G
Institute of Biological Chemistry, Washington State University, Pullman, Washington, United States of America.
Institute of Biological Chemistry, Washington State University, Pullman, Washington, United States of America ; Ealasid, Inc., Pullman, Washington, United States of America.
PLoS One. 2013 Dec 26;8(12):e83169. doi: 10.1371/journal.pone.0083169. eCollection 2013.
Fast growing hybrid poplar offers the means for sustainable production of specialty and commodity chemicals, in addition to rapid biomass production for lignocellulosic deconstruction. Herein we describe transformation of fast-growing transgenic hybrid poplar lines to produce 2-phenylethanol, this being an important fragrance, flavor, aroma, and commodity chemical. It is also readily converted into styrene or ethyl benzene, the latter being an important commodity aviation fuel component. Introducing this biochemical pathway into hybrid poplars marks the beginnings of developing a platform for a sustainable chemical delivery system to afford this and other valuable specialty/commodity chemicals at the scale and cost needed. These modified plant lines mainly sequester 2-phenylethanol via carbohydrate and other covalently linked derivatives, thereby providing an additional advantage of effective storage until needed. The future potential of this technology is discussed. MALDI metabolite tissue imaging also established localization of these metabolites in the leaf vasculature.
速生杂交杨树不仅为木质纤维素解构提供快速的生物质生产，还为特种和商品化学品的可持续生产提供了途径。在此，我们描述了将速生转基因杂交杨树品系进行转化以生产2-苯乙醇的过程，2-苯乙醇是一种重要的香料、调味剂、香气成分和商品化学品。它还可以很容易地转化为苯乙烯或乙苯，后者是一种重要的商品航空燃料成分。将这种生化途径引入杂交杨树标志着一个可持续化学输送系统平台开发的开端，该平台能够以所需的规模和成本提供这种及其他有价值的特种/商品化学品。这些改良的植物品系主要通过碳水化合物和其他共价连接的衍生物来隔离2-苯乙醇，从而提供了在需要之前有效储存的额外优势。本文还讨论了该技术的未来潜力。基质辅助激光解吸电离代谢物组织成像也确定了这些代谢物在叶片脉管系统中的定位。
