Maia Marisa, McCann Andréa, Malherbe Cédric, Far Johann, Cunha Jorge, Eiras-Dias José, Cordeiro Carlos, Eppe Gauthier, Quinton Loïc, Figueiredo Andreia, De Pauw Edwin, Sousa Silva Marta
Laboratório de FTICR e Espectrometria de Massa Estrutural, Faculdade de Ciências da Universidade de Lisboa, Lisboa, Portugal.
Grapevine Pathogen Systems Lab (GPS Lab), Biosystems and Integrative Sciences Institute (BioISI), Faculdade de Ciências da Universidade de Lisboa, Lisboa, Portugal.
Front Plant Sci. 2022 Oct 10;13:1012636. doi: 10.3389/fpls.2022.1012636. eCollection 2022.
Despite well-established pathways and metabolites involved in grapevine- interaction, information on the molecules involved in the first moments of pathogen contact with the leaf surface and their specific location is still missing. To understand and localise these molecules, we analysed grapevine leaf discs infected with with MSI. Plant material preparation was optimised, and different matrices and solvents were tested. Our data shows that trichomes hamper matrix deposition and the ion signal. Results show that putatively identified sucrose presents a higher accumulation and a non-homogeneous distribution in the infected leaf discs in comparison with the controls. This accumulation was mainly on the veins, leading to the hypothesis that sucrose metabolism is being manipulated by the development structures of . Up to our knowledge this is the first time that the localisation of a putatively identified sucrose metabolite was shown to be associated to infection sites.
尽管在葡萄相互作用中涉及的途径和代谢物已得到充分证实，但关于病原体与叶片表面接触初期所涉及的分子及其具体位置的信息仍然缺失。为了了解和定位这些分子，我们分析了感染微星的葡萄叶片圆盘。优化了植物材料的制备，并测试了不同的基质和溶剂。我们的数据表明，毛状体阻碍了基质沉积和离子信号。结果表明，与对照相比，推测鉴定出的蔗糖在受感染的叶片圆盘中积累更高且分布不均匀。这种积累主要集中在叶脉上，从而得出蔗糖代谢正被[未明确的结构]的发育结构所操纵的假设。据我们所知，这是首次表明推测鉴定出的蔗糖代谢物的定位与[未明确的感染源]感染部位相关。
