通过结构域内Kd/Dd重组体进行表位的精细定位。
Fine mapping of epitopes by intradomain Kd/Dd recombinants.
作者信息
Abastado J P, Jaulin C, Schutze M P, Langlade-Demoyen P, Plata F, Ozato K, Kourilsky P
出版信息
J Exp Med. 1987 Aug 1;166(2):327-40. doi: 10.1084/jem.166.2.327.
Abstract
11 intradomain recombinants between H-2Kd and H-2Dd were produced using an original technique based on in vivo recombination in Escherichia coli. After transfection into mouse L cells, all these recombinants were expressed at high levels on the cell surface. The specificities of 77 mAbs were examined on these cell lines. mAbs could be organized in 12 groups. In each group, a small number of amino acids participating in the recognized epitope(s) were identified. In a few instances, noncontinuous epitopes comprising amino acids belonging to different domains of the antigen were found. The data thus obtained are compatible with those produced in previous exon-shuffling experiments, but permit a much more precise definition of recognized epitope(s).
摘要
利用一种基于大肠杆菌体内重组的原创技术，产生了11个H-2Kd和H-2Dd之间的结构域内重组体。转染到小鼠L细胞后，所有这些重组体均在细胞表面高水平表达。在这些细胞系上检测了77种单克隆抗体的特异性。单克隆抗体可分为12组。在每组中，鉴定出了参与识别表位的少数氨基酸。在少数情况下，发现了由属于抗原不同结构域的氨基酸组成的不连续表位。由此获得的数据与先前外显子改组实验产生的数据相符，但能更精确地定义识别的表位。
相似文献
1
Fine mapping of epitopes by intradomain Kd/Dd recombinants.通过结构域内Kd/Dd重组体进行表位的精细定位。
J Exp Med. 1987 Aug 1;166(2):327-40. doi: 10.1084/jem.166.2.327.
2
Introduction of H-2Dd determinants into the H-2Ld antigen by site-directed mutagenesis.通过定点诱变将H-2Dd决定簇引入H-2Ld抗原。
J Exp Med. 1987 Sep 1;166(3):744-60. doi: 10.1084/jem.166.3.744.
3
Exon shuffling: mapping polymorphic determinants on hybrid mouse transplantation antigens.外显子改组：在杂种小鼠移植抗原上定位多态性决定簇
Nature. 1982 Dec 23;300(5894):755-7. doi: 10.1038/300755a0.
4
5
6
Expression and T cell recognition of hybrid antigens with amino-terminal domains encoded by Qa-2 region of major histocompatibility complex and carboxyl termini of transplantation antigens.具有由主要组织相容性复合体Qa-2区域编码的氨基末端结构域和移植抗原羧基末端的杂合抗原的表达及T细胞识别
J Exp Med. 1985 May 1;161(5):935-52. doi: 10.1084/jem.161.5.935.
7
Intradomain H-2Kd/Dd recombinants define the same regions as crucial for recognition by alloreactive or major histocompatibility complex-restricted cytolytic T cells.域内H-2Kd/Dd重组体确定了与同种异体反应性或主要组织相容性复合体限制的细胞溶解T细胞识别至关重要的相同区域。
Eur J Immunol. 1989 Jan;19(1):193-6. doi: 10.1002/eji.1830190131.
8
New family of exon-shuffled recombinant genes reveals extensive interdomain interactions in class I histocompatibility antigens and identifies residues involved.外显子重排重组基因新家族揭示了I类组织相容性抗原中广泛的结构域间相互作用，并确定了相关残基。
J Exp Med. 1987 Jan 1;165(1):211-22. doi: 10.1084/jem.165.1.211.
9
10
A monoclonal antibody recognizes a subset of the H-2Dd mouse major class I antigens.
Res Immunol. 1989 Jun-Aug;140(5-6):581-94. doi: 10.1016/0923-2494(89)90121-1.
引用本文的文献
1
Experimental Structures of Antibody/MHC-I Complexes Reveal Details of Epitopes Overlooked by Computational Prediction.抗体/MHC-I 复合物的实验结构揭示了计算预测忽略的表位细节。
J Immunol. 2024 Apr 15;212(8):1366-1380. doi: 10.4049/jimmunol.2300839.
2
Experimental structures of antibody/MHC-I complexes reveal details of epitopes overlooked by computational prediction.抗体/MHC-I复合物的实验结构揭示了计算预测所忽略的表位细节。
bioRxiv. 2023 Dec 4:2023.12.01.569627. doi: 10.1101/2023.12.01.569627.
3
Molecular analysis of TCR and peptide/MHC interaction using P18-I10-derived peptides with a single D-amino acid substitution.使用具有单个D-氨基酸取代的P18-I10衍生肽对TCR与肽/MHC相互作用进行分子分析。
Biophys J. 2007 Apr 1;92(7):2570-82. doi: 10.1529/biophysj.106.095208. Epub 2007 Jan 5.
4
Activating Ly-49D and inhibitory Ly-49A natural killer cell receptors demonstrate distinct requirements for interaction with H2-D(d).激活型Ly-49D和抑制型Ly-49A自然杀伤细胞受体与H2-D(d)相互作用表现出不同的要求。
J Exp Med. 2000 Aug 7;192(3):447-54. doi: 10.1084/jem.192.3.447.
5
The natural killer cell receptor Ly-49A recognizes a peptide-induced conformational determinant on its major histocompatibility complex class I ligand.自然杀伤细胞受体Ly-49A识别其主要组织相容性复合体I类配体上的肽诱导构象决定簇。
Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11792-7. doi: 10.1073/pnas.93.21.11792.
6
MHC class I molecules form ternary complexes with calnexin and TAP and undergo peptide-regulated interaction with TAP via their extracellular domains.MHC I类分子与钙连蛋白和抗原加工相关转运体（TAP）形成三元复合物，并通过其细胞外结构域与TAP进行肽调节的相互作用。
J Exp Med. 1996 Aug 1;184(2):337-48. doi: 10.1084/jem.184.2.337.
7
H-2Dd exploits a four residue peptide binding motif.H-2Dd利用一个四残基肽结合基序。
J Exp Med. 1993 Dec 1;178(6):1877-92. doi: 10.1084/jem.178.6.1877.
8
Cells expressing a major histocompatibility complex class I molecule with a single covalently bound peptide are highly immunogenic.表达带有单个共价结合肽的主要组织相容性复合体I类分子的细胞具有高度免疫原性。
J Exp Med. 1995 Feb 1;181(2):493-502. doi: 10.1084/jem.181.2.493.
9
Dimerization of soluble major histocompatibility complex-peptide complexes is sufficient for activation of T cell hybridoma and induction of unresponsiveness.可溶性主要组织相容性复合体-肽复合物的二聚化足以激活T细胞杂交瘤并诱导无反应性。
J Exp Med. 1995 Aug 1;182(2):439-47. doi: 10.1084/jem.182.2.439.
10
Single Dd amino acid substitutions in the H-2Ld molecule identify antibody epitopes.H-2Ld分子中的单个Dd氨基酸取代可识别抗体表位。
Immunogenetics. 1994;39(6):440-3. doi: 10.1007/BF00176164.
本文引用的文献
1
Monoclonal antibodies to mouse major histocompatibility complex antigens.针对小鼠主要组织相容性复合体抗原的单克隆抗体。
Transplantation. 1982 Sep;34(3):113-20. doi: 10.1097/00007890-198209000-00001.
2
DNA sequence of a gene encoding a BALB/c mouse Ld transplantation antigen.编码BALB/c小鼠Ld移植抗原的基因的DNA序列。
Science. 1982 Feb 5;215(4533):679-82. doi: 10.1126/science.7058332.
3
Genetics of the major histocompatibility complex: the final act.主要组织相容性复合体的遗传学：终曲。
Annu Rev Immunol. 1983;1:119-42. doi: 10.1146/annurev.iy.01.040183.001003.
4
Activation of a Qa/Tla class I major histocompatibility antigen gene is a general feature of oncogenesis in the mouse.Qa/Tla 一类主要组织相容性抗原基因的激活是小鼠肿瘤发生的一个普遍特征。
Nature. 1983;306(5945):756-60. doi: 10.1038/306756a0.
5
Formation of genes coding for hybrid proteins by recombination between related, cloned genes in E. coli.通过大肠杆菌中相关克隆基因之间的重组形成编码杂合蛋白的基因。
Nucleic Acids Res. 1983 Aug 25;11(16):5661-9. doi: 10.1093/nar/11.16.5661.
6
A cDNA clone containing the entire coding sequence of a mouse H-2Kd histocompatibility antigen.一个包含小鼠H-2Kd组织相容性抗原完整编码序列的cDNA克隆。
Nucleic Acids Res. 1983 Mar 11;11(5):1567-77. doi: 10.1093/nar/11.5.1567.
7
Sequencing end-labeled DNA with base-specific chemical cleavages.通过碱基特异性化学切割对末端标记的DNA进行测序。
Methods Enzymol. 1980;65(1):499-560. doi: 10.1016/s0076-6879(80)65059-9.
8
Dissection of serological and cytolytic T lymphocyte epitopes on murine major histocompatibility antigens by a recombinant H-2 gene separating the first two external domains.通过分离前两个外部结构域的重组H-2基因剖析小鼠主要组织相容性抗原上的血清学和细胞溶解性T淋巴细胞表位
J Exp Med. 1984 Jul 1;160(1):167-78. doi: 10.1084/jem.160.1.167.
9
Domain interactions of H-2 class I antigens alter cytotoxic T-cell recognition sites.H-2 Ⅰ类抗原的结构域相互作用改变细胞毒性T细胞识别位点。
Nature. 1984;309(5965):279-81. doi: 10.1038/309279a0.
10
Murine H-2Dd-reactive monoclonal antibodies recognize shared antigenic determinant(s) on human HLA-B7 or HLA-B27 molecules or both.鼠源H-2Dd反应性单克隆抗体可识别人类HLA - B7或HLA - B27分子或两者上的共同抗原决定簇。
Immunogenetics. 1983;17(4):357-70. doi: 10.1007/BF00372455.
Zotero 插件