Sakuma K, Ohyama T, Sogawa K, Fujii-Kuriyama Y, Matsumura Y
J Nutr. 1987 Jun;117(6):1141-8. doi: 10.1093/jn/117.6.1141.
The effects of diet at the molecular level were investigated by feeding rats control or protein-deficient diets. Each of the control and the protein-deficient groups was further divided into three subgroups according to the level of energy intake. Liver DNA, RNA and total cellular protein concentrations and serum albumin and albumin mRNA concentrations were determined. Protein deficiency caused a marked inhibition of liver growth but the size of most cells remained normal. The low protein diet caused concurrent decreases in the levels of serum albumin and serum mRNA. However, this effect was observed only with the combination of a low protein diet and normal energy intake. The low protein-low energy diet failed to induce the low serum albumin level. Our findings suggest that the altered balance between at least two factors of protein and energy intake serves as a trigger for several metabolic changes which ultimately regulate specific genes.
通过给大鼠喂食对照饮食或蛋白质缺乏饮食，研究了饮食在分子水平上的影响。根据能量摄入水平，将对照组和蛋白质缺乏组各自进一步分为三个亚组。测定了肝脏DNA、RNA和总细胞蛋白浓度以及血清白蛋白和白蛋白mRNA浓度。蛋白质缺乏导致肝脏生长明显受抑，但大多数细胞的大小仍保持正常。低蛋白饮食导致血清白蛋白水平和血清mRNA水平同时下降。然而，这种效应仅在低蛋白饮食与正常能量摄入相结合时才观察到。低蛋白-低能量饮食未能诱导血清白蛋白水平降低。我们的研究结果表明，蛋白质和能量摄入的至少两个因素之间平衡的改变是引发多种代谢变化的触发因素，这些代谢变化最终调节特定基因。
