Esposito Debora, Chen Amelia, Grace Mary H, Komarnytsky Slavko, Lila Mary Ann
Plants for Human Health Institute, North Carolina State University , 600 Laureate Way, Kannapolis, North Carolina 28081, United States.
J Agric Food Chem. 2014 Jul 23;62(29):7022-8. doi: 10.1021/jf4051599. Epub 2014 Jan 17.
Wild lowbush blueberries (Vaccinium angustifolium Ait) are a rich source of anthocyanins and other flavonoids with anti-inflammatory activities; however, their individual effects on cellular signaling remain to be elucidated. This study determined the capacity of blueberry bioactives to protect murine RAW 264.7 macrophages from lipopolysaccharide-induced inflammation. Fractionation of the crude extract (CE) into polyphenol-rich (PPR), anthocyanin-rich (ANC), and proanthocyanidin-rich (PAC) fractions and an ethyl acetate fraction (EA) revealed that PPR, ANC, and PAC components most effectively suppressed mRNA biomarkers of acute inflammation (Cox-2, iNOS, and IL-1β). Among major polyphenols found in the wild blueberries, malvidin-3-glucoside was significantly more effective than epicatechin or chlorogenic acid in reducing the expression of pro-inflammatory genes in vitro.
野生矮丛蓝莓（Vaccinium angustifolium Ait）富含具有抗炎活性的花青素和其他类黄酮；然而，它们对细胞信号传导的个体影响仍有待阐明。本研究确定了蓝莓生物活性成分保护小鼠RAW 264.7巨噬细胞免受脂多糖诱导的炎症的能力。将粗提物（CE）分离为富含多酚（PPR）、富含花青素（ANC）和富含原花青素（PAC）的组分以及乙酸乙酯组分（EA），结果表明PPR、ANC和PAC组分最有效地抑制了急性炎症的mRNA生物标志物（Cox-2、iNOS和IL-1β）。在野生蓝莓中发现的主要多酚中，锦葵色素-3-葡萄糖苷在体外降低促炎基因表达方面比表儿茶素或绿原酸显著更有效。
