蓝莓中的多酚可调节 LPS 诱导的 RAW264.7 巨噬细胞中的炎症细胞因子。
Polyphenols from blueberries modulate inflammation cytokines in LPS-induced RAW264.7 macrophages.
机构信息
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Institute of Agro-food Science and Technology, Shandong Academy of Agriculture Science, Jinan 250100, China.
Institute of Agro-food Science and Technology, Shandong Academy of Agriculture Science, Jinan 250100, China.
出版信息
Int J Biol Macromol. 2014 Aug;69:382-7. doi: 10.1016/j.ijbiomac.2014.05.071. Epub 2014 Jun 3.
Polyphenols including 3-glucoside/arabinoside/galactoside-based polymers of delphinidins, petunidins, peonidins, malvidins and cyanidins are one type of biological macromolecules, which are extraordinarily rich in blueberries. Anti-inflammatory activity of blueberry polyphenols (BPPs) was investigated by using lipopolysaccharide (LPS) induced RAW264.7 macrophages. The results showed that BPPs suppressed the gene expression of IL-1β (interleukin-1β), IL-6 and IL-12p35. The inhibition effect on IL-1β and IL-6 mRNA was most obvious at the concentration of 10-200μg/mL BPPs. But the inhibition effect on IL-12p35 mRNA was increased with the increasing concentration of BPPs. When fixed at 100μg/mL BPPs, the most significant inhibition on IL-1β, IL-6 and IL-12p35 mRNA expression was detected at 12-48h. In conclusion, BPPs exhibit anti-inflammation activity by mediating and modulating the balances in pro-inflammatory cytokines of IL-1β, IL-6, and IL-12.
多酚包括飞燕草素、矮牵牛素、矢车菊素、锦葵素和花青素的 3-葡糖苷/阿拉伯糖苷/半乳糖苷基聚合物，是一类生物大分子，在蓝莓中含量非常丰富。通过脂多糖（LPS）诱导的 RAW264.7 巨噬细胞来研究蓝莓多酚（BPPs）的抗炎活性。结果表明，BPPs 抑制了白细胞介素-1β（IL-1β）、IL-6 和 IL-12p35 的基因表达。在 10-200μg/mL 的 BPPs 浓度下，对 IL-1β 和 IL-6 mRNA 的抑制作用最为明显。但对 IL-12p35 mRNA 的抑制作用随着 BPPs 浓度的增加而增加。当固定在 100μg/mL 的 BPPs 时，在 12-48h 检测到对 IL-1β、IL-6 和 IL-12p35 mRNA 表达的最显著抑制作用。综上所述，BPPs 通过调节和调节促炎细胞因子 IL-1β、IL-6 和 IL-12 的平衡发挥抗炎作用。
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