Department of Gastroenterology and Hepatology, Radboud University Nijmegen Medical Centre , Nijmegen, The Netherlands.
J Agric Food Chem. 2014 Jan 29;62(4):950-5. doi: 10.1021/jf403312v. Epub 2014 Jan 15.
Since the food matrix determines β-carotene availability for intestinal absorption, food matrix effects on the bioaccessibility of β-carotene from two diets were investigated in vitro and compared with in vivo data. The "mixed diet" consisted of β-carotene-rich vegetables, and the "oil diet" contained β-carotene-low vegetables with supplemental β-carotene. The application of extrinsically labeled β-carotene was also investigated. The bioaccessibility of β-carotene was 28 μg/100 μg β-carotene from the mixed diet and 53 μg/100 μg β-carotene from the oil diet. This ratio of 1.9:1 was consistent with in vivo data, where the apparent absorption was 1.9-fold higher in the oil diet than in the mixed diet. The labeled β-carotene was not equally distributed over time. In conclusion, the food matrix effects on bioaccessibility of β-carotene could be measured using an in vitro model and were consistent with in vivo data. The application of extrinsically labeled β-carotene was not confirmed.
由于食物基质决定了β-胡萝卜素在肠道吸收中的可用性，因此本研究采用体外法研究了两种饮食中β-胡萝卜素的食物基质效应，并与体内数据进行了比较。“混合饮食”由富含β-胡萝卜素的蔬菜组成，“油脂饮食”则包含β-胡萝卜素含量低的蔬菜，并添加了β-胡萝卜素。本研究还对外源标记的β-胡萝卜素的应用进行了研究。混合饮食中β-胡萝卜素的生物可利用率为 28μg/100μgβ-胡萝卜素，油脂饮食中β-胡萝卜素的生物可利用率为 53μg/100μgβ-胡萝卜素。该比值为 1.9:1，与体内数据一致，即油脂饮食中的表观吸收率比混合饮食高 1.9 倍。标记的β-胡萝卜素在不同时间的分布并不均衡。总之，该研究采用体外模型测量了β-胡萝卜素生物可利用率的食物基质效应，且与体内数据一致。外源标记β-胡萝卜素的应用并未得到证实。
