Dudani Jaideep S, Go Derek E, Gossett Daniel R, Tan Andrew P, Di Carlo Dino
Department of Bioengineering, University of California Los Angeles , 420 Westwood Plaza, 5121 Engineering V, Box 951600, Los Angeles, California 90095, United States.
Anal Chem. 2014 Feb 4;86(3):1502-10. doi: 10.1021/ac402920m. Epub 2014 Jan 16.
Precise spatiotemporal control of how particles and cells interact with reagents is critical for numerous laboratory and industrial processes. Novel tools for exerting this control at shorter time scales will enable development of new chemical processes and biomedical assays. Previously, we have developed a generalized approach to manipulate cells and particles across fluid streams termed rapid inertial solution exchange (RInSE), which utilizes inertial lift forces at finite Reynolds number and high Peclet number to transfer particles from an initial solution to another within a millisecond. Here, we apply these principles toward developing a continuous flow microfluidic platform that enables transient chemical treatments of cells and particles (on the order of 1 ms). We also demonstrate how the reactant stream can be employed as a diffusion barrier, preventing adverse reactions between coflowing solutions. In order to demonstrate the utility of the method, we applied it to various operations in molecular biology and automated cell staining including cell permeabilization, fluorescent staining, and molecular delivery to viable cells. We expect this method will enable previously unexplored studies of the dynamics of molecular events, improve uniformity of reactions carried on the surface of beads, and increase uniformity in cell-based assays through automation.
精确控制颗粒和细胞与试剂相互作用的时空特性，对众多实验室和工业过程至关重要。能够在更短时间尺度上实现这种控制的新型工具，将推动新化学过程和生物医学检测方法的发展。此前，我们开发了一种通用方法，用于在流体流中操控细胞和颗粒，称为快速惯性溶液交换（RInSE），该方法利用有限雷诺数和高佩克莱数下的惯性升力，在毫秒级时间内将颗粒从初始溶液转移到另一种溶液中。在此，我们运用这些原理开发了一个连续流微流控平台，可对细胞和颗粒进行短暂的化学处理（约1毫秒）。我们还展示了如何将反应物流用作扩散屏障，防止共流溶液之间发生不良反应。为了证明该方法的实用性，我们将其应用于分子生物学和细胞自动染色的各种操作，包括细胞通透化、荧光染色以及向活细胞进行分子递送。我们预计该方法将开启此前未被探索的分子事件动力学研究，提高珠粒表面反应的均匀性，并通过自动化提高基于细胞检测的均匀性。
