Jang Dong-Woo, Franco Rose Ann, Sarkar Swapan Kumar, Lee Byong-Taek
From the *Department of Biomedical Engineering and Materials, College of Medicine, Soonchunhyang University, Cheonan, Korea.
ASAIO J. 2014 Mar-Apr;60(2):216-23. doi: 10.1097/MAT.0000000000000032.
In this study, a novel porous hydroxyapatite scaffold was designed and fabricated to imitate natural bone through a multipass extrusion process. The conceptual design manifested unidirectional microchannels at the exterior part of the scaffold to facilitate rapid biomineralization and a central canal that houses the bone marrow. External and internal fissures were minimized during microwave sintering at 1,100 °C. No deformation was noted, and a mechanically stable scaffold was fabricated. Detailed microstructure of the fabricated artificial bone was examined by scanning electron microscope and X-ray diffractometer, and material properties like compressive strength were evaluated. The initial biocompatibility was examined by the cell proliferation of MG-63 osteoblast-like cells using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Preliminary in vivo investigation in a rabbit model after 4 weeks and 8 weeks of implantation showed full osteointegration of the scaffold with the native tissue, and formation of bone tissue within the pore network, as examined by microcomputed tomography analyses and histological staining. Osteon-like bone microarchitecture was observed along the unidirectional channel with microblood vessels. These confirm a biomimetic regeneration model in the implanted bone scaffold, which can be used as an artificial alternative for damaged bone.
在本研究中，设计并制造了一种新型多孔羟基磷灰石支架，通过多道挤压工艺模拟天然骨。概念设计显示，支架外部有单向微通道以促进快速生物矿化，还有一个容纳骨髓的中央管。在1100℃微波烧结过程中，将外部和内部裂缝降至最低。未观察到变形，制造出了机械稳定的支架。通过扫描电子显微镜和X射线衍射仪检查制造的人工骨的详细微观结构，并评估其抗压强度等材料性能。使用MTT（3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐）法通过MG-63成骨样细胞的细胞增殖来检查初始生物相容性。植入4周和8周后在兔模型中进行的初步体内研究表明，通过微计算机断层扫描分析和组织学染色检查，支架与天然组织完全骨整合，并且在孔隙网络内形成了骨组织。沿着带有微血管的单向通道观察到了类骨单位的骨微结构。这些证实了植入骨支架中的仿生再生模型，其可作为受损骨的人工替代品。
