Li Yi-Chao, Luo Jian-Min, Lu Rong-Wen, Liu Kai-Mao, Levy Alexander Miles, Yao Xin-Cheng
Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
Department of Ophthalmology and Visual Sciences, University of Nebraska Medical Center, Omaha, NE 68138, USA.
J Mod Opt. 2012 Jun 20;59(11). doi: 10.1080/09500340.2012.687464.
Dynamic monitoring of stimulus-evoked inner neural response is important for functional validation of stimulation protocols of retinal prosthetic devices. In this paper, we demonstrate label-free intrinsic optical signal (IOS) imaging of electrically stimulated inner neural response in freshly isolated mouse retinas. While single-pulse stimulation evoked rapid IOS within 20 ms, pulse-train stimulation indicated that the fast IOS response can follow frequency stimulation up to at least 8 Hz. Fast IOS imaging promises a noninvasive method for high resolution examination of electrically evoked retinal response, without artifact contamination of electrical stimulus.