Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA.
Opt Lett. 2010 Jun 1;35(11):1810-2. doi: 10.1364/OL.35.001810.
Understanding of visual signal processing can benefit from simultaneous measurement of different types of retinal neurons working together. In this Letter, we demonstrate that intrinsic optical signal (IOS) imaging of frog retina slices allows simultaneous observation of stimulus-evoked responses propagating from the photoreceptors to the inner neurons. High-resolution imaging revealed robust IOSs at the photoreceptor, the inner plexiform, and the ganglion cell layers. While IOSs of the photoreceptor layer were mainly confined to the area directly stimulated by the visible light, IOSs of the inner retinal layers spread from the stimulus site into relatively large areas with a characteristic near-to-far time course.
对视觉信号处理的理解可以受益于同时测量协同工作的不同类型的视网膜神经元。在这封信件中，我们证明了青蛙视网膜切片的固有光学信号 (IOS) 成像可以同时观察从光感受器到内神经元传播的刺激诱发反应。高分辨率成像显示在光感受器、内丛状和神经节细胞层具有强大的 IOS。虽然光感受器层的 IOS 主要局限于可见光直接刺激的区域，但内视网膜层的 IOS 从刺激部位传播到具有特征性近到远时程的相对较大区域。
