Li Yi-Chao, Cui Wan-Xing, Wang Xu-Jing, Amthor Franklin, Lu Rong-Wen, Thompson Anthony, Yao Xin-Cheng
Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
Opt Express. 2011 Jan 3;19(1):99-106. doi: 10.1364/OE.19.000099.
Simultaneous monitoring of many functioning β-cells is essential for understanding β-cell dysfunction as an early event in the progression to diabetes. Intrinsic optical signal (IOS) imaging has been shown to allow high resolution detection of stimulus-evoked physiological responses in the retina and other neural tissues. In this paper, we demonstrate the feasibility of using IOS imaging for functional examination of insulin secreting INS-1 cells, a popular model for investigating diabetes associated β-cell dysfunction. Our experiments indicate that IOS imaging permits simultaneous monitoring of glucose-stimulated physiological responses in multiple cells with high spatial (sub-cellular) and temporal (sub-second) resolution. Rapid IOS image sequences revealed transient optical responses that had time courses tightly correlated with the glucose stimulation.
同时监测多个功能正常的β细胞对于理解β细胞功能障碍这一糖尿病进展早期事件至关重要。固有光信号（IOS）成像已被证明能够高分辨率检测视网膜和其他神经组织中刺激诱发的生理反应。在本文中，我们证明了使用IOS成像对胰岛素分泌INS-1细胞进行功能检查的可行性，INS-1细胞是研究糖尿病相关β细胞功能障碍的常用模型。我们的实验表明，IOS成像能够以高空间（亚细胞）和时间（亚秒级）分辨率同时监测多个细胞中葡萄糖刺激的生理反应。快速的IOS图像序列揭示了与葡萄糖刺激时间进程紧密相关的瞬态光学反应。
