一种在动物模型中测定急性毒性的新方法。
A new method for determining acute toxicity in animal models.
作者信息
Chinedu Enegide, Arome David, Ameh Fidelis Solomon
机构信息
Department of Science Laboratory Technology (Physiology and Pharmacology Technology), University of Jos, Jos, Nigeria.
出版信息
Toxicol Int. 2013 Sep;20(3):224-6. doi: 10.4103/0971-6580.121674.
BACKGROUND
The intake of pharmacological substances by man has solely increased and this may be in the form of food, medicines and beverages, other industrial and household products. These substances are capable of eliciting chronic and acute toxicity, which may be mild or severe, depending upon their nature. Acute toxicity is defined as the unwanted effect(s) that occurs either immediately or at a short time interval after a single or multiple administration of such substance within 24 hours. The principal aim of this paper is to introduce a new method for testing toxicity, which if adopted, should produce more accurate and reproducible results using few animals.
MATERIALS AND METHODS
The proposed method is divided into three stages, with the outcome of each stage determining whether to terminate testing of proceed to the next stage. A confirmatory (confidence) test is used to validate the final test result. The method shows enormous advantages, which include the use of few animals, exploration of a wide range of doses, it is simple and inexpensive.
CONCLUSION
Furthermore, accurate and reproducible result can be gotten through this method. We therefore recommend that the method should be considered for endorsement for the testing of acute toxicity by the regulatory bodies.
背景
人类摄入的药物性物质一直在增加，其形式可能为食物、药品、饮料以及其他工业和家用产品。这些物质能够引发慢性和急性毒性，毒性的轻重取决于其性质。急性毒性被定义为在24小时内单次或多次施用此类物质后立即或在短时间间隔内出现的不良效应。本文的主要目的是介绍一种新的毒性测试方法，若采用该方法，使用较少动物就能得出更准确且可重复的结果。
材料与方法
所提出的方法分为三个阶段，每个阶段的结果决定是终止测试还是进入下一阶段。采用验证性（置信度）测试来验证最终测试结果。该方法具有诸多优点，包括使用动物数量少、能探索广泛的剂量范围、操作简单且成本低廉。
结论
此外，通过该方法能够获得准确且可重复的结果。因此，我们建议监管机构应考虑认可该方法用于急性毒性测试。
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