BioMicroSystems Laboratory, School of Electronic and Computing Systems, University of Cincinnati, Cincinnati, Ohio 45221, USA.
Biomicrofluidics. 2013 Aug 21;7(4):44119. doi: 10.1063/1.4818906. eCollection 2013.
In this paper, we report an inertial microfluidic device with simple geometry for continuous extraction of large particles with high size-selectivity (<2 μm), high efficiency (∼90%), and high purity (>90%). The design takes advantage of a high-aspect-ratio microchannel to inertially equilibrate cells and symmetric chambers for microvortex-aided cell extraction. A side outlet in each chamber continuously siphons larger particles, while the smaller particles or cells exit through the main outlet. The design has several advantages, including simple design, small footprint, ease of paralleling and cascading, one-step operation, and continuous separation with ultra-selectivity, high efficiency and purity. The described approach is applied to manipulating cells and particles for ultra-selective separation, quickly and effectively extracting larger sizes from the main flow, with broad applications in cell separations.
本文报道了一种具有简单几何形状的惯性微流控装置，用于连续高效（~90%）、高纯度（>90%）地高选择性（<2μm）提取大颗粒。该设计利用高纵横比微通道实现细胞的惯性平衡，并利用微涡旋辅助细胞提取的对称腔室。每个腔室的侧出口连续虹吸较大的颗粒，而较小的颗粒或细胞则通过主出口排出。该设计具有几个优点，包括设计简单、占地面积小、易于并联和级联、一步操作以及具有超选择性、高效率和高纯度的连续分离。所描述的方法可用于超选择性分离细胞和颗粒，从主流中快速有效地提取较大尺寸的颗粒，在细胞分离中有广泛的应用。
